LOCUS       sequence-369551-        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLentiGuide modified to express mCherry and a non-targeting sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Stransky LA, Vigeant SM, Huang B, West D, Denize T, Walton E, 
            Signoretti S, Kaelin WG Jr
  TITLE     Sensitivity of VHL mutant kidney cancers to HIF2 inhibitors does not
            require an intact p53 pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Apr 5;119(14):e2120403119. doi: 
            10.1073/pnas.2120403119. Epub 2022 Mar 31.
  PUBMED    35357972
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-04-5"; volume: "119(14):e2120403119. 
            doi"; pages: " 10.1073/pnas.2120403119. Epub 2022 Mar 31"
COMMENT     Sequence Label: pLentiGuide_mCherry_NT135
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        87..1265
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          318..1256
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1213..1233
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1278..1874
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1278..1297)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1774..1794
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1884..1940
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(2083..2101)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    2664..3252
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2717..2737)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3135..3146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3324..3557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3635..3756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3672..3691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3726..3745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3796..3931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3951..3970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3952..3970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3980..3997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3980..3996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3989..4011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4138..4593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4225..4244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4435..4456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4619..4723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4724..4792,4793..5584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4942..4961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5755..6343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6244..6263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6497..6514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6631..6652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6667..6672,6673..6690,6691..6697)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6705..6721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6710..6732
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6729..6745
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6729..6745
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6764..6784
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6766..6784
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6812..7038
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7039..7219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7266..7391
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7884..8117
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8302..8346
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8524..8772
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8524..8544
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8695..8714
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8794..8869
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8925..9042
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      421 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      481 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      541 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      601 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      661 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      721 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      781 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
      841 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
      901 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
      961 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1021 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1081 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1141 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1201 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1261 cgtgacgtac ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     1321 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     1381 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     1441 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg
     1501 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     1561 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     1621 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc
     1681 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     1741 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     1801 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     1861 gcaagcccgg tgccggtagt ggtgcgacta attttagctt attaaaacag gctggtgacg
     1921 tagaggaaaa tcccggtcct gtgagcaagg gcgaggagga taacatggcc atcatcaaag
     1981 agtttatgcg gttcaaggtg cacatggaag gcagcgtgaa cggccacgag ttcgagatcg
     2041 agggcgaggg cgaaggcagg ccctacgagg gcacccagac cgccaagctg aaggtgacca
     2101 agggcggacc tctgcccttc gcctgggaca tcctgagccc ccagttcatg tacggcagca
     2161 aggcctacgt gaagcacccc gccgacatcc ccgactacct gaagctgtcc ttccccgagg
     2221 gcttcaagtg ggagcgggtg atgaacttcg aggacggcgg ggtggtgacc gtgacccagg
     2281 atagctccct gcaggacggc gagttcatct acaaggtgaa actgcggggc accaacttcc
     2341 ccagcgacgg ccccgtgatg cagaaaaaga caatgggctg ggaggccagc agcgagcgga
     2401 tgtaccccga ggatggcgcc ctgaagggcg agatcaagca gcggctgaag ctgaaggacg
     2461 gcggccacta cgacgccgag gtgaaaacca cctacaaggc caaaaagccc gtgcagctgc
     2521 ctggcgccta caacgtgaac atcaagctgg atatcaccag ccacaacgag gactacacca
     2581 tcgtggagca gtacgagcgg gccgagggca gacacagcac cggcggcatg gacgagctgt
     2641 acaagtaaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     2701 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2761 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2821 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2881 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2941 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3001 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3061 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3121 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3181 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3241 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     3301 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3361 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3421 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3481 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3541 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     3601 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     3661 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3721 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     3781 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3841 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3901 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     3961 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4021 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4081 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     4141 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4201 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4261 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4321 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4381 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4441 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4501 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4561 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     4621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5881 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6001 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6061 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6361 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6421 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6481 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6541 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6601 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6661 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6721 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     6781 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     6841 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     6901 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     6961 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7021 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7081 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7141 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7201 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7261 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     7321 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     7381 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     7441 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     7501 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     7561 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     7621 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     7681 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     7741 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7801 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7861 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7921 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7981 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8041 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8101 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8161 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8221 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8281 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     8341 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     8401 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     8461 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     8521 agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     8581 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     8641 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     8701 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     8761 ggacgaaaca ccgcgcttcc gcggcccgtt caagttttag agctagaaat agcaagttaa
     8821 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct
     8881 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     8941 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9001 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9061 agcagagatc cactttggcg ccggctcgag gggg
//
