LOCUS       sequence-369580-        8656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA plasmid with neomycin resistance expressing a single guide RNA 
            targeting mGreenLantern and EGFP fluorescent proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Madireddy I, Pierson Smela M
  TITLE     Stably Integrating an Inducible CRISPR-Cas9 to Protect Against Viral
            Infections in Vitro.
  JOURNAL   MicroPubl Biol. 2022 Jun 16;2022. doi: 
            10.17912/micropub.biology.000590. eCollection 2022.
  PUBMED    35789697
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2022-06-16"; volume: "2022. doi"; pages: " 
            10.17912/micropub.biology.000590. eCollection 2022"
COMMENT     Sequence Label: rtTA-sg-mGreenLantern/EGFP
FEATURES             Location/Qualifiers
     source          1..8656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        107..355
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     107..127
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        376..451
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        482..1693
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1530..1549
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(1752..2369,2370..2375,2376..2411,2412..2414,2415..2450,
                     2451..2453,2454..2489,2490..2498)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2536..3107
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2701..2718)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2928..2947
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3123..3917
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3123..3917
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3177..3196)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3787..3806
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3959..4080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3996..4015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4050..4069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4843..4877
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5368..5384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5368..5384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5381..5403)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5392..5408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5416..5422,5423..5440,5441..5446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5461..5482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5599..5616)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5770..6358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5850..5869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6529..7320,7321..7389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7152..7171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7390..7494)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7520..7975)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7657..7678)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7869..7888
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8102..8124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8116..8133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8117..8133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8263..8325
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
       61 ggggatacgg ggaaaaggcc tccacggccc cataaagatg gttaatgagg gcctatttcc
      121 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
      181 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
      241 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttatcgtaac
      301 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggca
      361 cgggcagctt gccgggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      421 tcaacttgaa aaagtggcac cgagtcggtg ctttttttcc gcattaaccc acccaagatc
      481 tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct
      541 gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca
      601 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
      661 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
      721 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
      781 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
      841 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
      901 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
      961 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
     1021 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
     1081 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     1141 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     1201 tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag
     1261 ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt
     1321 cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt
     1381 gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa
     1441 tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc
     1501 ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg
     1561 gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc
     1621 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
     1681 ttttttgtta gacgaagctt ggtaccgagc tcggatctcc accccgtacc ggtcctgcag
     1741 tcgaattcac catgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac
     1801 tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     1861 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     1921 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     1981 actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg
     2041 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     2101 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     2161 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     2221 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     2281 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     2341 tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact
     2401 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     2461 ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat ccgcggccgc
     2521 actagaggaa ttccgcccct ctcccccccc cccctaacgt tactggccga agccgcttgg
     2581 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     2641 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     2701 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     2761 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     2821 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     2881 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     2941 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     3001 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     3061 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct taccggtcca
     3121 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3181 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3241 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3301 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3361 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3421 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3481 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     3541 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     3601 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     3661 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3721 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3781 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3841 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3901 ttgacgagtt cttctgacaa tcaacctctg gattacaaaa tttgtggtta actaactaaa
     3961 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4021 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4081 tcatgtctgg aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc
     4141 cttccggggg acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt
     4201 cttatatccc ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag
     4261 tccctaaaga aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag
     4321 ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     4381 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     4441 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     4501 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     4561 ctgtccctga tatctataac aagaaaatat atatataata agttatcacg taagtagaac
     4621 atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc
     4681 atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg
     4741 acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg
     4801 gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc
     4861 agactatctt tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc
     4921 ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct
     4981 tttcccgcga ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg
     5041 acgttgagcg aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct
     5101 ttaacggtga actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga
     5161 cacagttccg gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc
     5221 ggaactgccg tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca
     5281 gcgaggacgg gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac
     5341 ccggcgggcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5401 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5461 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5521 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5581 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5641 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5701 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5761 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5821 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5881 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5941 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6001 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6061 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7501 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7561 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7621 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7681 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7741 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7801 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7861 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7921 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     7981 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8041 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8101 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt
     8161 gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag
     8221 atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat
     8281 attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg
     8341 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     8401 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca
     8461 aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa
     8521 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     8581 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     8641 cgggcacggg gaaggt
//
