LOCUS       sequence-369583-        8656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA plasmid with neo resistance expressing a double cutting single 
            gRNA targeting tdTomato fluorescent protein sites 90.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Madireddy I, Pierson Smela M
  TITLE     Stably Integrating an Inducible CRISPR-Cas9 to Protect Against Viral
            Infections in Vitro.
  JOURNAL   MicroPubl Biol. 2022 Jun 16;2022. doi: 
            10.17912/micropub.biology.000590. eCollection 2022.
  PUBMED    35789697
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2022-06-16"; volume: "2022. doi"; pages: " 
            10.17912/micropub.biology.000590. eCollection 2022"
COMMENT     Sequence Label: rtTA-sg-tdTomato90/816
FEATURES             Location/Qualifiers
     source          1..8656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        157..405
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     157..177
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        426..501
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        532..1743
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1580..1599
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(1802..2419,2420..2425,2426..2461,2462..2464,2465..2500,
                     2501..2503,2504..2539,2540..2548)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2586..3157
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2751..2768)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2978..2997
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3173..3967
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3173..3967
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3227..3246)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3837..3856
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4009..4130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4046..4065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4100..4119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4893..4927
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5418..5434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5418..5434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5431..5453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5442..5458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5466..5472,5473..5490,5491..5496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5511..5532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5649..5666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5820..6408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5900..5919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6579..7370,7371..7439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7202..7221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7440..7544)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7570..8025)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7707..7728)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7919..7938
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8152..8174
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8166..8183
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8167..8183
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8313..8375
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat
       61 cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg
      121 ggaaaaggcc tccacggccc cataaagatg gttaatgagg gcctatttcc catgattcct
      181 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
      241 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
      301 gcagttttaa aattatgttt taaaatggac tatcatatgc ttatcgtaac ttgaaagtat
      361 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggcca cgagttcgag
      421 atcgagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      481 aaagtggcac cgagtcggtg ctttttttcc gcattaaccc acccaagatc tggcctccgc
      541 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
      601 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
      661 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
      721 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
      781 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
      841 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
      901 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
      961 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     1021 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     1081 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     1141 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     1201 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     1261 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     1321 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     1381 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     1441 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     1501 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     1561 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     1621 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     1681 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     1741 gacgaagctt ggtaccgagc tcggatctcc accccgtacc ggtcctgcag tcgaattcac
     1801 catgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt
     1861 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     1921 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     1981 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     2041 gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     2101 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     2161 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     2221 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     2281 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     2341 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     2401 gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct
     2461 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
     2521 ttttgacctt gacatgctcc ccgggtaact aagtaaggat ccgcggccgc actagaggaa
     2581 ttccgcccct ctcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     2641 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     2701 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     2761 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     2821 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     2881 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     2941 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3001 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     3061 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     3121 cggggacgtg gttttccttt gaaaaacacg atgataagct taccggtcca ccatgattga
     3181 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3241 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3301 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     3361 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     3421 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     3481 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     3541 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     3601 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     3661 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     3721 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     3781 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     3841 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     3901 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     3961 cttctgacaa tcaacctctg gattacaaaa tttgtggtta actaactaaa cttgtttatt
     4021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     4141 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     4201 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     4261 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     4321 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     4381 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     4441 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     4501 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     4561 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     4621 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     4681 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     4741 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     4801 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     4861 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     4921 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     4981 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     5041 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     5101 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     5161 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     5221 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     5281 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     5341 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     5401 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5461 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5521 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5581 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5641 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5701 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5821 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6241 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7561 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7621 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7681 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7741 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7801 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7861 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     7921 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7981 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     8041 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     8101 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8161 gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt gaacccgtgg
     8221 aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag atgaagatgc
     8281 tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat attgtgacgt
     8341 acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
     8401 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
     8461 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca aaaactcaaa
     8521 atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa agcccccagg
     8581 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
     8641 ggtccggcgc tccccc
//
