LOCUS       sequence-369587-        8657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA plasmid with neo resistance expressing a double cutting single 
            gRNA targeting tdTomato fluorescent protein sites 166.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Madireddy I, Pierson Smela M
  TITLE     Stably Integrating an Inducible CRISPR-Cas9 to Protect Against Viral
            Infections in Vitro.
  JOURNAL   MicroPubl Biol. 2022 Jun 16;2022. doi: 
            10.17912/micropub.biology.000590. eCollection 2022.
  PUBMED    35789697
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2022-06-16"; volume: "2022. doi"; pages: " 
            10.17912/micropub.biology.000590. eCollection 2022"
COMMENT     Sequence Label: rtTA-sg-tdTomato166/892
FEATURES             Location/Qualifiers
     source          1..8657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        271..346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        377..1588
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1425..1444
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(1647..2264,2265..2270,2271..2306,2307..2309,2310..2345,
                     2346..2348,2349..2384,2385..2393)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2431..3002
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2596..2613)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2823..2842
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3018..3812
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3018..3812
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3072..3091)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3682..3701
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3854..3975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3891..3910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3945..3964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4738..4772
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5263..5279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5263..5279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5276..5298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5287..5303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5311..5317,5318..5335,5336..5341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5356..5377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5494..5511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5665..6253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5745..5764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6424..7215,7216..7284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7047..7066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7285..7389)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7415..7870)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7552..7573)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7764..7783
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7997..8019
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8011..8028
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8012..8028
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8158..8220
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttatcg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg atgtcccagg cgaagggcag gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttccgcatt
      361 aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
      421 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
      481 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
      541 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
      601 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
      661 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
      721 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
      781 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
      841 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
      901 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
      961 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     1021 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     1081 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     1141 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     1201 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     1261 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     1321 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     1381 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     1441 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     1501 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     1561 ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc
     1621 gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg
     1681 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     1741 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     1801 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     1861 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     1921 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     1981 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     2041 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     2101 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     2161 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     2221 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     2281 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     2341 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     2401 aggatccgcg gccgcactag aggaattccg cccctctccc ccccccccct aacgttactg
     2461 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     2521 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     2581 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     2641 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     2701 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     2761 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     2821 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     2881 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     2941 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     3001 aagcttaccg gtccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3061 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3121 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3181 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3241 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3301 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3361 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3421 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3481 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3541 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3601 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3661 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3721 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3781 ccttctatcg ccttcttgac gagttcttct gacaatcaac ctctggatta caaaatttgt
     3841 ggttaactaa ctaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3961 catcaatgta tcttatcatg tctggaattg actcaaatga tgtcaattag tctatcagaa
     4021 gctcatctgg tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg
     4081 ttcccaaact gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc
     4141 ctcacaggaa cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact
     4201 ggattccttt tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc
     4261 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
     4321 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     4381 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     4441 ggctttgggg gggggctgtc cctgatatct ataacaagaa aatatatata taataagtta
     4501 tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc
     4561 acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg
     4621 acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct
     4681 aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat
     4741 gcgtcaattt tacgcagact atctttctag ggttaatcta gctgcatcag gatcatatcg
     4801 tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg
     4861 aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg
     4921 actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg
     4981 tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt
     5041 cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca
     5101 ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc
     5161 tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga
     5221 taccccgtga gttacccggc gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     5281 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5341 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5401 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5461 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5521 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5581 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5641 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5701 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5761 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5821 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5881 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5941 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6001 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6061 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6121 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6181 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6241 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6301 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6421 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7321 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7381 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     7441 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     7501 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7561 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     7621 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     7681 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     7741 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     7801 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     7861 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     7921 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     7981 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg
     8041 ttcattcacg tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac
     8101 agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc
     8161 tagaaagata atcatattgt gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg
     8221 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     8281 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     8341 ttattaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg
     8401 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     8461 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     8521 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     8581 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaggc ctccacggcc
     8641 ccataaagat ggttaat
//
