LOCUS       sequence-369589-        8657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA plasmid with neomycin resistance expressing a single guide RNA 
            targeting mGreenLantern fluorescent protein site 332..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Madireddy I, Pierson Smela M
  TITLE     Stably Integrating an Inducible CRISPR-Cas9 to Protect Against Viral
            Infections in Vitro.
  JOURNAL   MicroPubl Biol. 2022 Jun 16;2022. doi: 
            10.17912/micropub.biology.000590. eCollection 2022.
  PUBMED    35789697
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2022-06-16"; volume: "2022. doi"; pages: " 
            10.17912/micropub.biology.000590. eCollection 2022"
COMMENT     Sequence Label: rtTA-sg-mGreenLantern332
FEATURES             Location/Qualifiers
     source          1..8657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..290
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     42..62
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        312..387
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        418..1629
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1466..1485
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(1688..2305,2306..2311,2312..2347,2348..2350,2351..2386,
                     2387..2389,2390..2425,2426..2434)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2472..3043
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2637..2654)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2864..2883
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3059..3853
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3059..3853
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3113..3132)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3723..3742
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3895..4016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3932..3951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3986..4005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4779..4813
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5304..5320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5304..5320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5317..5339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5328..5344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5352..5358,5359..5376,5377..5382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5397..5418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5535..5552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5706..6294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5786..5805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6465..7256,7257..7325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7088..7107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7326..7430)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7456..7911)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7593..7614)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7805..7824
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8038..8060
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8052..8069
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8053..8069
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8199..8261
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tacggggaaa aggcctccac ggccccataa agatggttaa tgagggccta tttcccatga
       61 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
      121 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      181 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttatc gtaacttgaa
      241 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gtacctacaa
      301 gacccgcgcc ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      361 cttgaaaaag tggcaccgag tcggtgcttt ttttccgcat taacccaccc aagatctggc
      421 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
      481 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
      541 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
      601 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
      661 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
      721 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
      781 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
      841 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
      901 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
      961 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
     1021 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
     1081 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
     1141 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     1201 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
     1261 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
     1321 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
     1381 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     1441 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     1501 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     1561 tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt
     1621 ttgttagacg aagcttggta ccgagctcgg atctccaccc cgtaccggtc ctgcagtcga
     1681 attcaccatg tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa
     1741 tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     1801 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     1861 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     1921 tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa
     1981 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     2041 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     2101 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     2161 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     2221 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     2281 gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga
     2341 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
     2401 tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatccgc ggccgcacta
     2461 gaggaattcc gcccctctcc cccccccccc taacgttact ggccgaagcc gcttggaata
     2521 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2581 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2641 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2701 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2761 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     2821 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     2881 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     2941 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     3001 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttacc ggtccaccat
     3061 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3121 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3181 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3241 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     3301 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     3361 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     3421 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     3481 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     3541 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     3601 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     3661 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     3721 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     3781 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     3841 cgagttcttc tgacaatcaa cctctggatt acaaaatttg tggttaacta actaaacttg
     3901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4021 gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc
     4081 cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta
     4141 tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc
     4201 taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc
     4261 cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     4321 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     4381 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     4441 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     4501 ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     4561 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     4621 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     4681 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
     4741 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     4801 tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct
     4861 cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc
     4921 ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt
     4981 tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa
     5041 cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca
     5101 gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa
     5161 ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga
     5221 ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg
     5281 cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7441 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     7501 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     7561 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     7621 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     7681 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     7741 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     7801 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     7861 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     7921 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     7981 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     8041 agtcacgacg ttgtaaaacg acggccagtg agcgcgcctc gttcattcac gtttttgaac
     8101 ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga
     8161 agatgctcga cacgctgcag aacacgcagc tagattaacc ctagaaagat aatcatattg
     8221 tgacgtacgt taaagataat catgtgtaaa attgacgcat gtgttttatc ggtctgtata
     8281 tcgaggttta tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac
     8341 taataataaa ttcaacaaac aatttattta tgtttattta tttattaaaa aaaacaaaaa
     8401 ctcaaaattt cttctataaa gtaacaaaac ttttatgagg gacagccccc ccccaaagcc
     8461 cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc
     8521 cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg
     8581 cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct
     8641 gcagacacct gggggga
//
