LOCUS       sequence-369590-        5447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage Minorna.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_Minorna
FEATURES             Location/Qualifiers
     source          1..5447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..45
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    60..200
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(126..145)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     286..308
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(464..1342)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1369..1653
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1576..1595
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3564..3591
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3610..3701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3702..3770,3771..4562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3920..3939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4733..5321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5222..5241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
       61 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
      181 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      241 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      301 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga
      361 aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
      421 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
      481 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
      541 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
      601 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
      661 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
      721 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
      781 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
      841 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
      901 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
      961 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     1021 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     1081 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     1141 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     1201 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     1261 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     1321 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     1381 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
     1441 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     1501 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     1561 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1621 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
     1681 ttaaccatgg tgagcgttaa gaccgcgcgt cgtctgcgta tgctgaacca gaaactgacc
     1741 ggttacaacg cgaacccgcg tagcatcccg aaagaggagc gtgaggacat tgcgatgatg
     1801 atggcggcga ccctgagcaa cttccgtgaa tttgcgtaca tcggcatgcg tttcctgggt
     1861 tttaccctga ccgacatgca ggcggatatt gcggagtata tgcaaaaggg cccgcgtaaa
     1921 cgtatggtgg cggcgcagcg tggtgaagcg aaaagcaccc tggcggcgct gtatgcggtt
     1981 tggcgtctga tccaggacca aagctgccgt atcctgattg tgagcggcgc ggagaagcaa
     2041 gcgagcgatg ttgcgaacct gatcattcgt atgctggaaa cctggccgct gctgtgctac
     2101 ctgaaagcgg acccgacccg tggcgatcgt accagcttcg agggttatga cgttcactgc
     2161 gatctgaagc cgctggacaa aagcgcgagc gtggcgtgcg ttggcattac cgcgagcctg
     2221 cagggcaagc gtgcggatct gctgatcccg gacgatattg agaccaccaa aaacggtctg
     2281 acccagaccc aacgtgaaca gctgctgacc atcagcaagg acttcgcggc gatttgcacc
     2341 cacggcgata ccctgtacct gggtaccccg caaaccaagg acagcatcta taaaaccctg
     2401 ccgggtcgtg gctttgaagt gcgtgtttgg ccgggccgta ttccgagcgt tgaaatggag
     2461 gaacgttacg gtagcaccct ggcgccgtat atccgtgagc tgattgaacg tggctacaag
     2521 cgtaccggtt ttggtgtgga tggtaccctg ggcgagagca ccgataaggg tcgttatgac
     2581 gaagatgcgc tgatcgagaa agaactggat ttcggcccgg agggttttca gctgcaatac
     2641 atgctggaca ccaccctgag cgatcagatg cgtacccgta ttaaactgag cgacatgctg
     2701 gtgtacagcg gtagccaaga tagcagcccg gaaaccttta gctatatcgc ggaccgtcgt
     2761 tacctgtatc agcacgagca cgaaggcatt atgggtcagc aaatgtactt cccggcgttt
     2821 tatggcgata tgcacctgcc gtatcaacac aaagttctgg tggtggaccc ggcgggttgc
     2881 ggtggcgatg aagttagcta tgcggcgggt ggcgcggcga acagctatat ccacctgttc
     2941 agcgtgggtg gctttcaggg tggcgttagc accgaaaaca tcgacaaact gattgatctg
     3001 tgcgtggagc tggacatcag cgatatggtg gttgaaagca acatgggtca cggcaccgtt
     3061 agcatgctga ttctgaaccg tctgcgtgag cgtcacctgg cgggtatcgg cgtgcgtgac
     3121 attaacaaca ccacccagaa ggaacgtcgt atcattgata ccatcagccc ggttacccgt
     3181 cgtcaccgtc tggtggttca cgaacgtgcg atccacgacg atattggcac ctgcatgctg
     3241 tacccgcgtg accgtcgttg gctgtatagc gcgttcgcgc aactggcggg catcacctac
     3301 gaccgtggta gcctggcgaa agacgatcgt gcggatgcga ttgcgatgat ggtggcggtt
     3361 ctgaacggtc acctggtgga ggacgaaaag gtggttgcgg cgcgtgagag cgaaaaacag
     3421 gcgctggcgt ttatcaacaa cccgctggat tgggcgcaag gcatggtgca gaagcaactg
     3481 cgtggtgttg cggcgcgtct gcagggtcgt ggtcgtggta aacaacaccg tggtcgtcgt
     3541 taatgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     3601 acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3661 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3721 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3781 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3841 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3901 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     3961 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4021 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4081 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4141 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4201 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4261 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4321 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4381 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4441 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4501 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4561 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4621 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4681 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4741 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4801 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4861 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4921 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4981 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5041 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5101 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5161 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5221 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5281 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5341 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5401 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgatacc
//
