LOCUS       sequence-369591-        5399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage PGT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_PGT2
FEATURES             Location/Qualifiers
     source          1..5399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    36..176
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(102..121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     262..284
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(440..1318)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1345..1629
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1552..1571
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3492..3519
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3538..3629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3630..3698,3699..4490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3848..3867)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4661..5249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5150..5169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
       61 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
      121 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
      181 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      241 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      301 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg
      361 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa
      421 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac
      481 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
      541 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
      601 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
      661 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
      721 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
      781 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
      841 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
      901 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
      961 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     1021 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     1081 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     1141 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     1201 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     1261 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     1321 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     1381 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa
     1441 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     1501 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     1561 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     1621 tttctccata cccgtttttt gggctaacag gaggaattaa ccatggcgct gcgtgagagc
     1681 gcggaacgtg aggagcaacg tctgctggag ctggcggcgc tgcgtgatat gtacccgacc
     1741 ttcctgccgt ttctgatcga cttcatggtt atgctgggct tctttgtgac cccggttcag
     1801 aagctgattg cgcaatttct ggaatacggc ccgctgtatc gtatggtgca ggcgcaacgt
     1861 ggtcaggcga aaaccaccat caccgcggcg tatgcgatct ggcgtctgat tcacgacacc
     1921 aacctggcgg tgctgattgt tagcgcgggt ggcaagcaag cgaacgagat cagcaccctg
     1981 attgttcgta gcttcaagac ctgggacaaa ctggcgtgca tgtgcccgga tgcgagcatg
     2041 ggtgatcgtg agagcgtgga agcgtttgat gttcactaca acctgaagcg tctgaacaaa
     2101 agcccgagcg tggcgtgcac cggtatcacc ggtaacctgc agggcaagcg tgcgggtctg
     2161 ctgattgcgg acgatgttga gagccagaag aacagcctga ccgcggtgat gcgtgaaaac
     2221 ctgctgaacc tgacccgtga tttcaccagc atctgcagca ccggcgacat catttacctg
     2281 ggtaccccgc agaccaccga aagcatctat aacaccctgc cgggccgtgg tttcaccgtt
     2341 cgtatttggc cgggccgttt tccgaccgcg gagcaggttg aagcgtacgg tgaccaactg
     2401 tgcccggtgt ttcgtatgaa aattgcgagc cgtccggacc tgaccaccgg ttatggtccg
     2461 gatggtctgc aaggtgcgcc ggtggacccg gttatcctgg gtgaagcgtt cctgaacaag
     2521 aaagaaattg accagggtac cgcgtacttt cagctgcaac acatgctgca aacccgtctg
     2581 atggatgcga tgcgtaagcc gctgcacgcg gagaaactga tcgttgcgca gctgccgaag
     2641 cgtgacggtc tgttcccgat caccattacc cgtgaaatta gcgttcaagc gtacatgcgt
     2701 cgtaacgtgc tgagcaagcc gctggtgttc ggcgttccgg gtagcgtgag caaagatgtt
     2761 gcgaccctgc agggcgtgtg cttttatatc gatccggcgg gtggcggtaa aaacgcggat
     2821 gagaccgcgt ttgcgtgcac cggttttctg aacagcaaca ttttcctgct ggattggggc
     2881 ggtctgccgg gcggttacgc gccggatgtt atgagcgcgc tggcgcaacg tgtgtttgat
     2941 gttcgtccga acgtggttaa gatcgagaaa aacttcggct atggtgcgtt tcgtgaagtg
     3001 tggctgccgt acctgtgggc gatttatgac aaggcgggcg tgaaccgtcc gggtgttgag
     3061 gacgatatgg tgcacggtca gaaggagaaa cgtatcattg acaccctgga accggttatg
     3121 ggccgtggta gcctgatcgt ggatgaacgt ctgattaaag aggacgaaga tcagaccatg
     3181 cgttacagcg cgcaaatgcg tgttacctat agcggcttcc tgcaaatggt gaacatcagc
     3241 tacgacaaag atgcgctggt tcacgacgat cgtctggatg cgctggaggg tgcggtgcgt
     3301 cactggcagg attggctggc gaagaaccaa gagaaagcga ttcaggcgca gcgtaagcgt
     3361 gaattcgagg aatggcagaa agacccgatg atgaagaaac gttgccaaag cgtgaaggaa
     3421 tataacgcgg gccgtcgtgg taaaaccatg tttgataagc gtaaacgtta atgccaggca
     3481 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt
     3541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3601 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3661 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3721 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3781 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3841 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     3901 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3961 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4021 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4081 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4141 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4201 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4261 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4321 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4381 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4441 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4801 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5281 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5341 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgag
//
