LOCUS       sequence-369592-        5441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage ECBP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_ECBP5
FEATURES             Location/Qualifiers
     source          1..5441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     485..504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     738..755
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    770..910
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(836..855)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     996..1018
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1174..2052)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2079..2363
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2286..2305
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      4268..4295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4314..4405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4406..4474,4475..5266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4624..4643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5437..5441,1..584)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
       61 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      121 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
      181 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      241 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      301 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      361 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      421 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      481 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      541 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      601 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      661 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      721 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
      781 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
      841 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
      901 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
      961 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     1021 ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg
     1081 gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc
     1141 cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt
     1201 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
     1261 tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
     1321 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct
     1381 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca
     1441 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
     1501 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
     1561 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
     1621 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
     1681 ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg
     1741 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
     1801 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac
     1861 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata
     1921 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
     1981 cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg
     2041 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
     2101 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac
     2161 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa
     2221 caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact
     2281 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc
     2341 gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa ttaaccatgc
     2401 gtcgtgtgaa ccgtaacgac aagaaagcgc agctggagat gtgggaggaa ctggaagcga
     2461 tccagaaggc gttcccgttt accgttcaag gtctgtggct gttcgcgagc ctggtgctgg
     2521 agaaaaccat cgttggcagc ccgcacctga accgtaccca gaaggatatt ctgagctttc
     2581 tgctgaacgg tggcaaatat atcggtattc aagcgagccg tggcatgacc aagaccgtga
     2641 tggcggcgat cctgtgctgc ttctgcctga ttcacatgcc gcactaccgt atcattgttt
     2701 ttagccagaa cggcaagcgt gcgaaagaaa tcgcgggctg ggtggttaag attttctatg
     2761 cgatcgacat tctggaagtg ctgcaaccgg acacctacgc gggtgatcgt agcagcatcg
     2821 aagcgttcga cattcactgg tgctttcgtg gcgcggataa aagcccgagc gttacctgct
     2881 acagcattga gagcggtgcg caaggtgcgc gtgcggatct gatcctggcg gacgatattg
     2941 aaagcctgca gaacagccgt accgcgcaaa gccgtgagtg gctgctggaa cagagcctgg
     3001 agtttgaaag cattaaccaa tatggtcgta tcatttacct gggtaccccg caaggcgtgg
     3061 agagcatcta taacaccctg ccggcgcgtg gttacgttat gcgtatttgg ccgggccgtt
     3121 atccgaccgc ggagcagctg gaattctacg gtaacgcgct ggcgccgctg tttcgtaagg
     3181 acatcgaagc ggatccgagc ctgcaaagcg gtggcggtct ggacggtacc atgggcaaac
     3241 cgaccacccc ggcgatgtac gatgaggaac tgctgcagga gaaggaaatg cgtcaaggtc
     3301 tggcgaaatt catgctgcag tttatggtgc acaccggcat gagcgaccgt gatcgtttca
     3361 agctgaaact ggagaacctg gttctgatga actttaacgc gatgcagggt gcggtgatgc
     3421 cggtttggaa cagcgatcaa cgtagcatgt ggcaagcggc gccgcgtttt ggtacccgtc
     3481 cgcaagatcg tttctatttt gcgatgcagc aaccgtacat catgcgtgac ttcgatctga
     3541 ccgtgatgtt tattgatccg gcgggcggtg gcagccgtag ccaggatgag atgggttatg
     3601 cgatcaccaa gctgctgggc acctacgttt atatttacga cgtggatggt gttaagggtg
     3661 gctacgagca gagcgaactg atgaaactgg tgcaagcggc gaaggacgcg aaagtgaacg
     3721 tttgctatgt tgagaagaac tacggtaacg gcgcgcactt cgcgatgctg aagccgctgt
     3781 tcgagaaatt ttacccggaa tgccgtctgg aggaaatcaa cagcaccggt cagaaggagc
     3841 tgcgtatcat tgataccctg gaaccgctgc tgggtcagca ccgtctggtg gttagccaaa
     3901 ccgcggtgat gaaagattat aacagcatcc agaagtaccc ggcggaagtg aaaatgacct
     3961 atagctgctt ttaccaactt gcgcacatta ccagcgaccg tggtgcgctg cgtcatgacg
     4021 atcgtctgga tgcgctggcg ggtgcggtgg cgcagatcgt taactatatt gactacgatc
     4081 aggagaaggc ggaaaaagac cgtcaagcgg aggcggataa acacttcttt gaagtgatgg
     4141 cgaacccgaa cggtttcgtt cacaccatga tgcacggtgg cgactttatg agctatgtgg
     4201 aacagcaaaa caccggcgcg aacgcgctga agaaatactt caactaatgc caggcatcaa
     4261 attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt
     4321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4381 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4441 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4501 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4561 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4621 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     4681 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4741 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4801 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4861 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4921 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4981 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5041 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5101 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5161 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5221 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5281 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5341 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga g
//
