LOCUS       sequence-369593-        5486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage SP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_SP6
FEATURES             Location/Qualifiers
     source          1..5486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     404..421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    436..576
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(502..521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     662..684
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(840..1718)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1745..2029
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1952..1971
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3979..4006
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4025..4116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4117..4185,4186..4977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4335..4354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5148..5486,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      421 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
      541 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
      601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
      661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag
      721 atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat ggacgaagca
      781 gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat
      841 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc
      901 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca
      961 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg
     1021 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
     1081 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
     1141 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
     1201 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
     1261 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
     1321 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat
     1381 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
     1441 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
     1501 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
     1561 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
     1621 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
     1681 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
     1741 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
     1801 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
     1861 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
     1921 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
     1981 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
     2041 gggctaacag gaggaattaa ccatggcgcg tgcgcgtgaa agccaagcgg aggcgctggc
     2101 gcgttgggaa gcgctgcacg agctgcagca aacctttccg tacaccgtgg cgggtctgct
     2161 gagctttgcg caggtggtta tcaacaacct gattaccggt aacccggacc tgaaccgtgt
     2221 gcaagcggat atcctgaagt tcctgtttgg tggcaacaaa tatcgtatgg ttgaagcgca
     2281 gcgtggtcaa gcgaagacca ccatcgcggc gatttacgcg gtttttcgta tcattcacga
     2341 gccgcacaag cgtatcatga ttgtgagcca gaccgcgaaa cgtgcggagg aaatcgcggg
     2401 ctgggttatc aagatttttc gtggtctgga cttcctggaa tttatgctgc cggacattta
     2461 cgcgggcgat aaggcgagca tcaaaggttt cgagattcac tataccctgc gtggcagcga
     2521 caaaagcccg agcgtggcgt gctacagcat tgaagcgggt atgcagggtg cgcgtgcgga
     2581 tatcattctg gcggacgatg ttgaaagcct gcaaaacagc cgtaccgcgg cgggtcgtgc
     2641 gctgctggag gacctgacca aagagtttga aagcatcaac cagttcggcg atatcattta
     2701 cctgggtacc ccgcaaagcg ttaacagcat ttacaacaac ctgccggcgc gtggctatca
     2761 gatccgtatt tggccgggtc gttacccgac cctggagcaa gaagcgtgct atggcgactt
     2821 cctggcgccg atgatccgtc aggatatgat tgacgatccg agcctgcgta gcggctatgg
     2881 tattgatggt acccaaggtg cgccgacctg cccggaaatg tacgacgatg agaagctgat
     2941 cgagaaagaa attagccagg gtaccgcgaa gttccagctg caatttatgc tgaacacccg
     3001 tctgatggac gcggatcgtt atccgctgcg tctgaaccaa ctgatcctga tgagctttgg
     3061 caccgacgtg gttccggaga tgccgacctg gagcaacgac agcgtgaacc tgattagcga
     3121 tgcgccgcgt ttcggtaaca aaccgaccga ttacctgtat cgtccggttc cgcgtccgta
     3181 cgaatggcgt ccgatccagc gtcgtctgat gtatattgac ccggcgggtg gcggtaaaaa
     3241 cggcgatgag accggtgtgg cgatcgtttt cctgctgggc accttcatct acgtgtacaa
     3301 ggttttcggt gtgccgggcg gttacagcga aagcgcgctg agccgtatcg tgcgtgaggc
     3361 gaagcaggcg gaagtgaaag aagtgttcat cgaaaagaac tttggccacg gtgcgttcga
     3421 ggcggttatt aaaccgtact tcgagcgtga atggccggcg gagctgaagg aagactatgc
     3481 gaccggtcaa aaagaagcgc gtatcattga gaccctggaa ccgctgatga gcgcgcaccg
     3541 tatcattttt aacgcggaaa tgatcaagca ggacattgat agcgttcaac actacccgct
     3601 ggaagtgcgt atgagctata gcctgttcgc gcagatgagc aacatcaccc tggagaaagg
     3661 ctgcctgcgt cacgacgatc gtctggatgc gctgtacggt gcgatccgtc agctgaccag
     3721 ccaaattgac tatgatgaag cgaaccgtat taaccgtctg cgtgcgaagg agatgcgtga
     3781 atatctggag atgatgaccg acccgctgcg tcgtcgtgag ttctttaccg gccaggatca
     3841 cggttaccgt aaaagcacca acgttagcaa cgcgatgcaa agccgtgtgt tcggcggtag
     3901 ccgtgtgaag gttaaaagcc gtaacaccat cagcagccgt attagccgta cctggtaatg
     3961 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     4021 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4081 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4141 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4201 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4261 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4321 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     4381 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4441 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4501 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4561 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4621 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4681 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4741 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4801 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4861 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4921 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4981 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5041 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5101 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5161 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5221 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5281 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5341 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5401 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5461 ggctgaacgg ggggttcgtg cacaca
//
