LOCUS       sequence-369598-        6464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses green adenosine indicator AdoLightG in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Kagiampaki Z, Rohner V, Kiss C, Curreli S, Dieter A, Wilhelm M, 
            Harada M, Duss SN, Dernic J, Bhat MA, Zhou X, Ravotto L, Ziebarth T,
            Wasielewski LM, Sonmez L, Benke D, Weber B, Bohacek J, Reiner A, 
            Wiegert JS, Fellin T, Patriarchi T
  TITLE     Sensitive multicolor indicators for monitoring norepinephrine in 
            vivo.
  JOURNAL   Nat Methods. 2023 Sep;20(9):1426-1436. doi: 
            10.1038/s41592-023-01959-z.
  PUBMED    37474807
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2023-09"; volume: "20"; issue: "9"; pages: 
            "1426-1436"
COMMENT     Sequence Label: pAAV_hSyn1_AdoLightG
FEATURES             Location/Qualifiers
     source          1..6464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        164..611
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          635..767
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     750..769
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1713..1734
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1840..1861)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2101..2120)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2843..3431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2896..2916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3314..3325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3449..3673
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3716..3856
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3716..3845
                     /label=AAV2 ITR
     rep_origin      3931..4386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4018..4037)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4228..4249
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4403..4422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4522..4544
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4582..4600)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4668..4772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4773..4841,4842..5633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4991..5010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5804..6392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6293..6312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(6454..6464,1..119)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgcacg cgtgtgtcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta
      181 ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca
      241 agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca
      301 ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc
      361 cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg
      421 gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag
      481 ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg
      541 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc
      601 tgagagcgca gtcgagatta attaactcga gcaggtaagt atcaaggtta caagacaggt
      661 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
      721 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg ctagcgttta
      781 aacggtaccg gatccgccac catgaagacg atcatcgccc tgagctacat cttctgcctg
      841 gtgttcgccg actacaagga cgatgatgac gcacccataa tgggctcatc cgtttacatc
      901 actgtcgaac ttgccattgc cgttctggcg attttgggaa atgtactggt ttgctgggct
      961 gtttggttga actccaactt gcagaacgtc actaactact tcgtcgtgag tcttgctgcc
     1021 gcggacatag cggtcggggt acttgccatc ccctttgcca taactatctc aactgggttt
     1081 tgtgcggctt gtcacggatg tctgttcata gcctgtttcg tgctcgttct tactcaatca
     1141 tccatattca gcctgcttgc gattgcaatt gatcggtact gggctatctc cagccctgcc
     1201 cggtatgaga gaaagatgac ccccaaggca gccttcatca taattgccat atgctgggtg
     1261 ttgagctttg cgattgggct gacaccgatg cttggttgga ataactgcgg acaaccgaag
     1321 gagggtaaga accactctca aggatgcgga gagggacagg ttgcatgcct cttcgaagat
     1381 gtcgtcccca tgaactacat ggtctatttc aatttcttcg cctgcgttct tgtgccactg
     1441 cttcttatgc tcggggtcta tctcagaatt ttcaggattg ctcagaaaca gctgagctca
     1501 ctcattaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttcaag
     1561 atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc
     1621 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccaaa
     1681 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1741 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg
     1801 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1861 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag
     1921 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1981 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2041 gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
     2101 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2161 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2221 gagtacaaca atcatgacca actgaaaaga gaaactaaag tcgccaagtc cttggcgatc
     2281 atagtaggtt tgtttgcgct ctgctggctt ccccttcata tcattaactg ttttacgttc
     2341 ttctgccccg actgcagcca tgcgccattg tggcttatgt acctcgcaat tgtcctctct
     2401 catacgaata gtgttgtgaa cccatttata tacgcgtata ggataaggga atttagacag
     2461 acgtttcgca aaataatcag atcccatgta ttgagacagc aagaaccttt taaggcagct
     2521 ggtaccagcg caagggtcct cgctgcacat gggagtgacg gcgaacaggt gagtctgagg
     2581 cttaatggtc accctcccgg ggtgtgggca aatggtagtg cgcctcatcc tgagcggcgg
     2641 ccgaatgggt acgccttggg tctggtatca ggcgggagtg ctcaggaatc tcagggcaac
     2701 actggacttc cggacgtaga actcttgagt catgagctca aaggagtgtg cccagaacca
     2761 cctgggctgg atgatcccct tgcacaggat ggagctggtg tctcataaga attctgtaca
     2821 gagcggccgc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2881 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2941 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3001 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3061 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3121 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3181 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3241 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3301 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3361 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3421 cgcctccccg ctctcgagat acgtacgact gtgccttcta gttgccagcc atctgttgtt
     3481 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3541 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3601 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3661 gtgggctcta tggcttctga ggcggaaaga tctcacgtgc ggaccgagcg gccgcaggaa
     3721 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     3781 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     3841 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3901 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     3961 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     4021 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4081 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4141 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4201 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4261 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     4321 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4381 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4441 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4501 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4561 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4621 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4681 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4741 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4801 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4861 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4921 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4981 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5041 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5101 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5161 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5221 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5281 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5341 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5401 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5461 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5521 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5581 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5641 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5941 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6421 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcag
//
