LOCUS       sequence-369614-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConLS and ConR1 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT057
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(36..624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(116..135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        727..831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(832..900,901..1692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1050..1069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
       61 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      541 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      601 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      661 cagtggaacg aaaactcacg ttaagggatt ttggtcatga caggtggcac ttttcgggga
      721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      841 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      901 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      961 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1021 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1081 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1141 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1201 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1261 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1321 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1381 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1441 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1501 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc ccgcggtatc
     1561 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1621 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1681 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1741 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatcgcgg ccgctaactg
     1801 ccttgatctg tcgggtgtct gcttctctca caggcttagg tggatctgct gtccccagtc
     1861 agtgcggtca tcatgcagtc atccgagcgt gtattgcaca catttgtttg gcgcatgatg
     1921 agccgtgatg accccgtgca gcaaacgagg accgattagc tgcaggacta ctagtagctc
     1981 gtctcattgg ctatgcctgc gcgtaccgcc acggtagcat cagatgcgga tatcagtgag
     2041 acgcagttct agaacgtgaa ttcgacctag ccacatttcc aaggcgtatt gcgacgaatt
     2101 gccacgttgt cgcagtgtct gtactgtggg ttaatgtctg tgatcgccgt cgtgagttcg
     2161 tgtcatccgc gcatgattca gatggtcctg gagatcgttg cttgtaccgg ctgtttgtgg
     2221 ctctaccagc ggccgcacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2281 gagcaaaagg ccagcaaaag
//
