LOCUS       sequence-369615-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL1 and ConRE connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT058
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(201..992,993..1061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     824..843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1062..1166)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1269..1857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1758..1777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcataccga tgactgcctg gcgactcaca actaagcaag acagccggaa ccagcgccgg
       61 cgaacaccac tgcatatatg gcatatcaca acagtccagc ggccgcgatt atcaaaaagg
      121 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      181 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      241 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      301 gagggcttac catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct
      361 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      421 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      481 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      541 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      601 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      661 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      721 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      781 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      841 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      901 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      961 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1021 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1081 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1141 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc
     1201 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1261 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1321 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1381 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1441 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1501 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1561 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1621 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1681 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1741 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1801 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1861 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1921 tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg ccgcacgggg tcatcacggc
     1981 tcatcatgcg ccaaacaaat gtgtgcaata cacgctcgga tgactgcatg atgaccgcac
     2041 tgactgggga cagcagatcc acctaagcct gtgagagaag cagacacccg acagatcaag
     2101 gcagttagcg gacccgatga actacagaag aattcactgt ctagaagctc gtctcaccaa
     2161 cagttggtgt taaggggtcc cctgcggtcc caacccgtct acagcatgag acgagtcact
     2221 agtatcgctg cagggaccag catattcatg tcagcgaaat ctgctcgtca gtggtgctca
     2281 cactgacgaa tcatgtacag
//
