LOCUS       sequence-36962-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene was not detected in the brain and 
            eye using LacZ. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1701
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     138..160
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      171..180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          230..362
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ccaggcaaag cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
       61 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
      121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acgggatccg ccatggtggc
      181 ggaagttcct ataccttttg aagaatagga acttcggaat aggaacttcc tgtggagaga
      241 aaggcaaagt ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc
      301 tgtctcgaca agcccagttt ctattggtct ccttaaacct gtcttgtaac cttgatactt
      361 acggcgcgcc acgcgtctgc tgcagcctcc tccagacgcg ccgccgcctc cggttgctgc
      421 acccagctcg gagcccgcag ttttcaccgg ggatggagga gagggggagc ggggagccgg
      481 gggctcagcc aatcagaggc ggagattggg ggagaaagga cggcagggtg gggtgggggg
      541 tggggaagga agagaaggcg gtcccgcatt ggtgtgagag tggcaggttg cggcggaagg
      601 gggccctcac cctgtggctg gcaggcctgg ccagggacgg gagggttcgg gaccccgggg
      661 atcagggtgc ggcagcccaa agaacattat cctcctccct gagccccgca atggttgttc
      721 cccttttaag gctttaaccc tttcttctga cctattcttc aggagtctgt gggaaaacca
      781 aagtgtaaga ggtgcaggat gcaggccggg agcgcccacg cctccacatt tccttttcag
      841 ctggagccca cctcctccgg
//
