LOCUS       sequence-369655-        5270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from Desulfurococcales archaeon ex4484_217_2 
            prophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_prophage_Desulfurococcales_archaeon
FEATURES             Location/Qualifiers
     source          1..5270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      106..694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     595..614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     848..865
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    880..1020
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(946..965)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1106..1128
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1284..2162)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2189..2473
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2396..2415
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      4207..4234
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4253..4344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4345..4413,4414..5205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4563..4582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
       61 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      241 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      301 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      361 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      421 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      481 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      541 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      601 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      661 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      721 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      781 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      841 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
      901 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      961 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     1021 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     1081 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     1141 tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcataa
     1201 tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg
     1261 tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct
     1321 tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag
     1381 aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg
     1441 gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta
     1501 atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt
     1561 gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc
     1621 tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc
     1681 agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc
     1741 ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg
     1801 cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg
     1861 cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag
     1921 tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt
     1981 ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt
     2041 cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc
     2101 gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc
     2161 atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat
     2221 tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt
     2281 aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc
     2341 tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc
     2401 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct
     2461 actgtttctc catacccgtt ttttgggcta acaggaggaa ttaaccatgc gtgagctgaa
     2521 cgaggaatat ctgagcgtgc tgattaacaa cttcaccgag tacctgggct acgtttatca
     2581 ctacatcggt ctgccggaac cgaccccgct gcagatccgt attagccaaa ttctgggcga
     2641 gaacccggat cgtctgatcc tggaagcggc gcgtggtacc ggcaagagct ggattgcggc
     2701 gatctacacc acctggcgtc tgattcgtaa catcgaggaa aagatcctga ttgtgagcgc
     2761 gagcggcccg aaaagcgtgg agatcgcgac cttcgttcgt agcctgtttg agaacgttcc
     2821 gctgctggaa cacctgaagc cgaccgcgga cagccgtgat agcgtgctga gcttcgatgt
     2881 tgcgggttgc aaaccggcga ttgcgccgag cgtgagcgcg ctgggcgtta ccagccagat
     2941 taccggcaag cgtgcgagcc tggtgatcgc ggacgatgtg gaagttccga gcaacagcat
     3001 gaccgagctg atgcgtgaaa aactgattca gcgtacccaa gagtttgaag cgctgctgat
     3061 tccggacatc ccgagcagca tcatttatct gggcaccccg cagagcctgg agagcatcta
     3121 taacaagctg gaataccgta ccatcattct gccggcggaa gtgccggata gcgaggaagt
     3181 ttacgaaggt aaactggacg attggattct gatgcaaggt ccggcgggta ccccgaccga
     3241 cccggtgcgt ttcccgaaag aggtgctgct ggaacgtaaa gcgggtatgg gcccggttgg
     3301 ctataagctg cagtacatgc tggacaccac cctgagcgat gcggagcgtt atccgctgaa
     3361 gcaaaaagac ctgattgtgc tgggtctgga tggcaaagag ggtccggcgg ttatcaccta
     3421 taccggtaac cgtgaatacg cgctgaagga tctgatgaac ttcggcttta ctggtgacgt
     3481 gtttcacaaa ccgcgtcgtg ttagcgagga atatctgccg tacgaccgta tcgtgatgag
     3541 cgttgatccg agcggtagcg gtgaagatga aaccgcgtac gcgatcattg gcgtgctgag
     3601 cggtaacgtt ttcctgctgg attggggtgg caccaagaaa ggctttagcg acgaagcgct
     3661 gctgatgctg gcgcagaagg cggcggaata taaagtgaac gagatcgttc cggaaaagaa
     3721 cttcggtagc ggcatgttca ccgagctgtt tcgtaaaatc ctgattccga tctacccgtg
     3781 caccatcatt gacgatttta acgtgcgtgg ccagaaggag aaacgtatca ttgataccct
     3841 ggcgccgctg atcggtaacc accaactggt ggttaacgaa gacaagattc aggaagaggc
     3901 gcgttgggtg caagagatcc cgagcgaacg tctggtttat agcctgttct accagatgac
     3961 ccacattacc tatgataaag gtagcattcc gcatgatgat cgtctggatg cgctggcgat
     4021 tgcgtgcagc tacgttagca acgacgtgat cgttgatgcg gaaaagcgtc tgcaagacat
     4081 gaaggagaaa gaatttgagg aatggctgaa cgcgaccatt tataacaaaa gcggccgtcg
     4141 tcacaacacc aagctgtgcg gtagcaccgc gaaaatctac cgttaatgcc aggcatcaaa
     4201 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     4261 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4321 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4381 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4441 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4501 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4561 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4621 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4681 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4741 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4801 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4861 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4921 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4981 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5041 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5101 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5161 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5221 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
//
