LOCUS       sequence-369657-        5360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from Sphingopyxis flava R11H prophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_prophage_Sphingopyxis_flava
FEATURES             Location/Qualifiers
     source          1..5360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    36..176
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(102..121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     262..284
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(440..1318)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1345..1629
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1552..1571
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3453..3480
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3499..3590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3591..3659,3660..4451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3809..3828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4622..5210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5111..5130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
       61 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
      121 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
      181 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      241 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      301 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg
      361 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa
      421 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac
      481 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
      541 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
      601 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
      661 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
      721 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
      781 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
      841 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
      901 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
      961 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     1021 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     1081 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     1141 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     1201 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     1261 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     1321 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     1381 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa
     1441 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     1501 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     1561 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     1621 tttctccata cccgtttttt gggctaacag gaggaattaa ccatgaccga ccagagcctg
     1681 accgcggagg gtcgtctgaa ggcgagcttc accctgtttc tgatctacgt gtgggtgcgt
     1741 gtgctgcgtc tgccgcgtcc gacccgtgtt caactggaca ttgcggatta tctggcgaac
     1801 ggcccgcgtc gtcgtttcat cgcggcgttt cgtggtgttg gcaaaacctt tctgagcgcg
     1861 gcgtacattg tgtggcgtct gtggaacaac ccggacgcga agatcatggt ggttagcgcg
     1921 aacgagcgtt tcgcggcgaa aattgcggcg tttatccaca ccatcattaa cgcggaggac
     1981 gtggttagcc tggagccggt gccgtggagc gagctgaagg cgcgtgcgaa gcagaaaaac
     2041 agcaccatgg aattcgatgt tggtccggcg atgccggaca aagattacag cgtgagcgcg
     2101 tttggtatta ccggtcaaat caccggtggc cgtgcggacc tgattctgtt cgacgatatc
     2161 gaggttccga acaacagcga aaccgaggcg atgcgtgaaa aactggtgga ccgtgcggcg
     2221 gaggcggcgg cgattctgaa accggatggt gaagtggttt atctgggcac cttccagagc
     2281 atggcgagca tctaccgtaa cctgcgtagc aagggttatg gtatgcgtct gtggccggcg
     2341 cgttacccga ccaaggacaa actgcacctg tatgaagata ccctggcgcc gctgctgaaa
     2401 gaggacatcg cgcaagatcc gggtctgatg accccgggtg cgagcagcct gggtggcgcg
     2461 ccgaccgacc cggcgcgttt taccgagctg gatctgatgg aacgtgaggt tgaatggggt
     2521 agcggtggct tccagctgca atttatgctg gacaccagcc tggtggacgc ggatcgtttc
     2581 ccgctgaaga cccgtgatct gatcattatg gacaccgcgc gtgatgttgc gccgatgcgt
     2641 gttgcgtggg gtagcgcgcc ggaacagctg gttaaggaca tggataacat tggtttcgac
     2701 ggcgatcgta tgtttcgtcc gatctacaaa agccaagaat gggttccgta taccggcagc
     2761 gtgctggaga ttgacccgag cggtaccggc accgatgaaa ccgcgtacgt tatcaccaag
     2821 ttcctgaacg gtcacgttta cgtgcgtaaa tggggtggct atgttgatgg tcacagcgat
     2881 gcgaccctgc gtgcgctggc ggagattgcg aaagaggaag aggtgaacct gatccgtgtg
     2941 gaagcgaact tcggtgacgg catgtttagc cgtctgctgg agccgcacct gcgtaagatc
     3001 ggttacattg ttccgatcga agatcacaaa gtgcacggca agaaagaaga gcgtatcatt
     3061 gcgaccgtgc gtccggcgct gcaggcgcac cgtattgtta tggacaccag cctggtgcgt
     3121 caagatctgg aagaggcgcg tagcggtagc cacgagggtc agggcgttag caaagcggag
     3181 cgtagcggtc tgtatcaact gacccatatg agcgcggcga agggtgcgct gcgtaaagac
     3241 gatcgtatcg acgtgctgag caacgcgctg gcgtactggg cggaatatat gggtgcggat
     3301 gcggaagagg cggagcgtcg tgcggaagcg aaggcgaccg cggagttcga aaaagcgctg
     3361 tttggcagcg cgattctgcc gatccacacc cgtccgaccc accgtgcgcg tggtcgtggc
     3421 cgtcgtcgtt aatgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     3481 tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     3841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3961 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4021 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4741 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5341 gccgcagccg aacgaccgag
//
