LOCUS       sequence-369658-        5246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from uncultured Caudovirales phage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_uncultured_phage
FEATURES             Location/Qualifiers
     source          1..5246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     438..457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     691..708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    723..863
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(789..808)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     949..971
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1127..2005)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2032..2316
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2239..2258
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      4026..4053
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4072..4163
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4164..4232,4233..5024)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4382..4401)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5195..5246,1..537)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
       61 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
      121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      601 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      661 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      721 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
      781 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
      841 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
      901 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
      961 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg
     1021 cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
     1081 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
     1141 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
     1201 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
     1261 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     1321 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     1381 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     1441 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     1501 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     1561 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     1621 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     1681 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     1741 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     1801 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     1861 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     1921 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     1981 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
     2041 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
     2101 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga
     2161 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
     2221 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
     2281 gacgcttttt atcgcaactc tctactgttt ctccataccc gttttttggg ctaacaggag
     2341 gaattaacca tgaacgagat cgacccgcgt ctgaaggatt tccgtaactt tctgtacatt
     2401 gtgtggcacc acctgggtct gccgaagccg accaaagttc agtacgacat cgcggattat
     2461 attcaaaacg gcccgcgtcg tagcgtggtt atggcgttcc gtggtgtggg caaaagctgg
     2521 atcaccagcg cgtatgtttg ccaccagctg ctgctggacc cgagcaagaa cattctggtg
     2581 gttagcgcga gcaaaggtcg tgcggacgat ttcagcacct ttaccctgcg tctgatccag
     2641 gagatcccga ttctgaacca cctgctgccg aaggaaagcc aacgtttcag caaagtggcg
     2701 tttgacgttg gtccggcgcc gccgcagcat gcgccgagcg tgaccagcaa gggtatcacc
     2761 agccaactga ccggcagccg tgcggatatc gttattgcgg acgatgtgga agttccgaac
     2821 aacagcgcga cccagaacat gcgtgagaag ctggcggaaa gcattaaaga gttcgaagcg
     2881 gtgctgaagc cgaacggtaa agttatcttt ctgggcaccc cgcaaaccga gagcagcatt
     2941 tacaacctgc tgccggaccg tggttataac atctgcattt ggccggtgcg tgcgccgagc
     3001 gaaaaagacc gtgttaacta cggcgatcgt ctggcgcgtt tcgtgagcag cctgaagccg
     3061 agcgagctgg ttgaaccgga ccgttttgat ggtaaagagc tgctggagcg tgaactgagc
     3121 tacggtcgta gcggctttag cctgcagtat atgctggaca ccagcctgag cgaccaagat
     3181 cgttacccgc tgaagatcaa cgacctgctg gtgatggatc tgaacaccga actggcgccg
     3241 cagaaactga tttgggcgag cggtaccgac caggcgatgc aagatctgca ctgcgttggt
     3301 ttcaacggcg atcgttacca ccgtccgatg gcgctggttg gtgaatggat gccgtataac
     3361 ggcagcgtta tggcgattga cccgagcggt cgtggcagcg atgaaaccgc gtacgcggtg
     3421 gttaagatgc tgaacggtaa cctgtttgtt accgatgcgg gtggcattgc gggtggctat
     3481 ggtcaggcgg ttctggataa actgggcgcg atcgcgaagg cgcagaaagt gaaccaaatt
     3541 ctggttgagg aaaacttcgg tcaaggcatg tttgcggagc tgctgaagcc ggtgctgacc
     3601 aaagttcacc cgtgcgcggt ggaactgacc cgtcacagca tccagaagga gaaacgtatc
     3661 attgacaccc tggaaccggt tatgaaccaa caccgtctgg tgttcgatgc gggtgttatt
     3721 aagcgtgact acaccagcac caaggatctg ccgaccgaga aagcgctgca ctaccagctg
     3781 atgtatcaaa tgagccgtgt gacccgtagc aaaggtgcgc tggcgcatga tgatcgtctg
     3841 gatgcgctgg cgatggcggt tgcgtattgg accgaacaga tggcgcaaga cgcggatcgt
     3901 aagatcgcga tgaaccgtag caaagcgatg gaccgtgagc tggaaaagtt tatgaacgcg
     3961 gtggttggtc gtaagccgca gggcgatacc tggatgaaag tgtaatgcca ggcatcaaat
     4021 taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat
     4081 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4141 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4201 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4261 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4321 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4381 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     4441 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4501 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4561 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4621 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4681 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4741 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4801 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4861 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4921 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4981 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5221 tctgctgctt gcaaacaaaa aaacca
//
