LOCUS       sequence-369659-        5276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from Ralstonia phage RsoP1EGY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_RsoP1EGY
FEATURES             Location/Qualifiers
     source          1..5276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..25
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    40..180
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(106..125)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     266..288
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(444..1322)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1349..1633
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1556..1575
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3373..3400
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3419..3510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3511..3579,3580..4371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3729..3748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4542..5130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5031..5050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
       61 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      121 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
      181 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
      241 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
      301 tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcataa
      361 tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg
      421 tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct
      481 tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag
      541 aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg
      601 gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta
      661 atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt
      721 gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc
      781 tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc
      841 agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc
      901 ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg
      961 cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg
     1021 cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag
     1081 tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt
     1141 ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt
     1201 cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc
     1261 gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc
     1321 atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat
     1381 tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt
     1441 aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc
     1501 tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc
     1561 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct
     1621 actgtttctc catacccgtt ttttgggcta acaggaggaa ttaaccatga ccaccaagca
     1681 gcacccggcg caaaaagact tccgtgtgtt catgtttatg gtttggcgtc atctgaacct
     1741 gccggagccg accccggtgc agtacgatat cgcgcactat ctgcaacacg gtccgcgtcg
     1801 tagcgtgatt gaagcgtttc gtggtgttgg caaaagctgg atcaccagcg cgctggtgtg
     1861 ctgggttctg tggaacgacc cgcagaagaa aattctggtg atcagcgcga gcaaggagcg
     1921 tgcggatgcg ttcagcacct ttgtgaaacg tctgattaac gaactgccgg ttctgcagca
     1981 cctgaagccg aaagcggacc aacgtgatag catgatcagc ttcgatgttg gtccggcgac
     2041 cccggatcac agcccgagcg tgaaaagcgt tggtattaac ggccagatca ccggcagccg
     2101 tgcggacatc attatcgcgg acgatgttga ggtgccgaac aacagcgcga cccaaatgat
     2161 gcgtgacaag ctgagcgagg cggtgaaaga aatggatgcg gttatcaagc cgctgcagac
     2221 cagccgtatt atctacctgg gtaccccgca aaccgaaatg agcctgtaca acgcgctgcc
     2281 ggagcgtggc tatgaagcgc gtatttggcc ggcgctgtac ccggagctgc acctggtggc
     2341 gaactataaa ggtcgtctgg cgccgttcat cacccgtgcg ctggaagcgg acaaaagcct
     2401 ggttggcgcg ccgaccgatc cgcgtcgttt taacgagacc gacctgctgg aacgtaaagc
     2461 gagctatggt cgtgcgggtt ttgcgctgca gtttatgctg gacaccagcc tgagcgacgg
     2521 tgatcgttat ccgctgaaaa ttgcggatct gatcgtgcaa aacctgaacc cgaccatggc
     2581 gcacgttaag attgcgtggg cggcggcgcc ggaagtgtgc atcaacgatc tgccggcggt
     2641 tgcgctgacc ggtgaccgtt actatcgtcc gatgtggacc gatcagcaaa tgagcgagta
     2701 caccggctgc gtgatggcga ttgatccgag cggtcgtggc gcggatgaaa ccggttatgc
     2761 gattatcaag atcctggcgg gtaacctgtt cctggttgcg gcgggtggcc tgagcggtgg
     2821 ctacagcgac gagaccctgg aaaccctggc gcgtctggcg aaaacccacc aggtgaacca
     2881 cgttattatc gaagcgaact ttggtgatgg catgtatacc aagctgatca ccccgttctt
     2941 tggcaaagtg ggccacaaag tgctggttga ggaagttaag cacagcaccc agaaagaggc
     3001 gcgtattatc gacaccctgg aaccggtgct gagcacccac cgtctgattg tggaccaaaa
     3061 ggttatcgag aacgatttcc gtaccgcgga acaggacatc aagtacagcc tgttttatca
     3121 aatgacccgt atcacccgtg acaagggtgc gctggcgcat gacgatcgtc tggatgcgct
     3181 ggcgattgcg gttgcgtact ggaccgagca catgagccgt gacaacgata aggcggcggc
     3241 ggcgatcaag gacaaagcgc tgaaagatga actgaagaaa tttgtgcacg gtgttctggg
     3301 cagcaagccg aaacgtacca gctggatgag cagcaacagc ggcagccgtt aatgccaggc
     3361 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
     3421 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3481 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3541 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3601 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3661 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3721 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     3781 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3841 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3901 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3961 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4021 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4081 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4141 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4201 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4261 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4321 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4381 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4441 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4501 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4561 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4621 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4681 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4741 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4801 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4861 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4921 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4981 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5041 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5101 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5161 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5221 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgac
//
