LOCUS       sequence-369660-        5354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage Pisces.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_Pisces
FEATURES             Location/Qualifiers
     source          1..5354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        826..1110
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1033..1052
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2928..2955
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2974..3065
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3066..3134,3135..3926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3284..3303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4097..4685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4586..4605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4839..4856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4871..5011
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4937..4956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5097..5119
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(5275..5354,1..799))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
       61 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
      121 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
      181 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
      241 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
      301 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
      361 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
      421 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
      481 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
      541 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
      601 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
      661 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
      721 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
      781 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc
      841 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac
      901 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
      961 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg
     1021 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct
     1081 ttttatcgca actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta
     1141 accatgagcc aggatctggc ggcgcgtcaa gcgctgatga ccgcgcgtat gaaagcggac
     1201 ttcgtgttct ttctgtttgt tctgtggaaa gcgctgagcc tgccggtgcc gacccgttgc
     1261 cagatcgata tggcgaagaa actgagcgcg ggtgacaacc gtcgtttcat tctgcaagcg
     1321 tttcgtggta tcggcaaaag cttcattacc tgcgcgtttg tggtttggaa gctgtggaac
     1381 aacccggacc tgaaattcat gatcgttagc gcgagcaagg aacgtgcgga tgcgaacagc
     1441 atctttatta agcgtatcat tgacctgatg ccgcagctgc aagagctgaa gccgaaacag
     1501 ggtcaacgtg atgcggtgat cagcttcgac gttggtccgg cgaaaccgga tcacagcccg
     1561 agcgtgaaga gcgttggtat taccggccaa ctgaccggta gccgtgcgga catcctgatt
     1621 gcggacgatg tggaagttcc gggtaacagc gcgacccaag cggcgcgtga tcgtctgagc
     1681 gagctggtga aggaatttga cgcgatcctg aaaccgggtg gcaccgttat ttacctgggt
     1741 accccgcaga ccgagatgac cctgtaccgt caactggaag gtcgtggcta tagcaccacc
     1801 atctggccgg cgcgttaccc gcgtgatgaa aaggactgga aaagctatgg tgatcgtctg
     1861 gcgccgatgc tgcaagcgga gctggaaagc gacccggagg gctactattg gctgccgacc
     1921 gatgaagtgc gtttcgacga tgaagacctg aaagagcgtg aactgagcta cggcaaggcg
     1981 ggcttcgcgc tgcaatttat gctgaacccg aacctgggtg atgcggaaaa gtatccgctg
     2041 aaactgcgtg acctgattgt ggcggatctg gacccggaga gcagcccgat ggtttaccag
     2101 tggctgccga acctgcaaaa caaacgtgac gatgtgccga acgttggtct gatgggcgac
     2161 agctaccaca cctatcagac cgtgggtagc gcgttcagca gctataccca aaaaatcctg
     2221 gttattgatc cgagcggtcg tggcaaggac gaaaccggtt acgcggtgct gtatcagctg
     2281 aacggctaca tctttgttat ggaggcgggt ggcatgcgtg gtggctatga ggatagcacc
     2341 ctggaagcgc tggcgaagat cggtcgtaag tggaaagtga acgagtacgt tattgaaggc
     2401 aacttcggtg acggcatgta tctggaactg tttaaaccgg tggcggcgcg tattcacccg
     2461 gcggcggtga ccgaggttaa gagcaagggt cagaaggaac tgcgtatctg cgatgttctg
     2521 gagccgatta tgggcagcca ccgtctgatc gtgaacagca gcaccattgt tagcgactac
     2581 cagaccgcgg cggataaaga cggtgtgcgt aacccgatct acagcctgtt ctatcaaatg
     2641 acccgtatta gccgtgagcg tggtgcgctg gcgcatgacg atcgtctgga tgcgctggcg
     2701 atcggcgtgc agttctttgt tgaaagcatg gcgaaggacg cggtgaaagg ccagcgtgag
     2761 gttaccgagg aatggctgga ggaacaaatg gaagatccga agaaaggttt taagagcatt
     2821 gagaccgact actgggcgaa cggcgtgcgt gttcagttca acaccgacga tgacctgggt
     2881 ctgggcagct atgttcaatt tcactaatgc caggcatcaa attaagcaga aggccatcct
     2941 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
     3001 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3061 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3121 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3181 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3241 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3301 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3361 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3421 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3481 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3541 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3601 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3661 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3721 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3781 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3841 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3901 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3961 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4021 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4081 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4141 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4201 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4261 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4321 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4381 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4441 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4501 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4561 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4621 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4681 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4741 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4801 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4861 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4921 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     4981 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     5041 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5101 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
     5161 ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt caaatggacg aagcagggat
     5221 tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga
     5281 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
     5341 ggccccggtg catt
//
