LOCUS       sequence-369664-        5351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_T7
FEATURES             Location/Qualifiers
     source          1..5351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..29
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    44..184
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(110..129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     270..292
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(448..1326)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1353..1637
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1560..1579
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3452..3479
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3498..3589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3590..3658,3659..4450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3808..3827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4621..5209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5110..5129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
       61 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
      121 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
      181 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
      241 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
      301 gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc
      361 ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa
      421 gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt
      481 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
      541 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
      601 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
      661 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg
      721 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
      781 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
      841 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc
      901 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
      961 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta
     1021 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc
     1081 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc
     1141 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt
     1201 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
     1261 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc
     1321 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
     1381 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
     1441 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
     1501 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
     1561 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
     1621 ctctactgtt tctccatacc cgttttttgg gctaacagga ggaattaacc atgagcaccc
     1681 agagcaaccg taacgcgctg gttgtggcgc aactgaaggg tgacttcgtg gcgttcctgt
     1741 ttgttctgtg gaaagcgctg aacctgccgg tgccgaccaa gtgccagatt gacatggcga
     1801 aagttctggc gaacggcgat aacaagaaat tcatcctgca agcgtttcgt ggtattggca
     1861 agagcttcat cacctgcgcg tttgtggttt ggagcctgtg gcgtgatccg cagctgaaga
     1921 ttctgatcgt gagcgcgagc aaagaacgtg cggacgcgaa cagcattttc atcaagaaca
     1981 tcattgatct gctgccgttt ctgagcgagc tgaaaccgcg tccgggtcag cgtgacagcg
     2041 tgattagctt cgatgttggt ccggcgaacc cggaccacag cccgagcgtg aagagcgttg
     2101 gtattaccgg ccaactgacc ggtagccgtg cggatatcat tatcgcggac gatgttgaaa
     2161 ttccgagcaa cagcgcgacc atgggtgcgc gtgagaagct gtggaccctg gtgcaggaat
     2221 ttgcggcgct gctgaaaccg ctgccgagca gccgtgttat ctacctgggt accccgcaaa
     2281 ccgagatgac cctgtacaaa gagctggaag acaaccgtgg ctataccacc attatctggc
     2341 cggcgctgta cccgcgtacc cgtgaggaaa acctgtacta tagccaacgt ctggcgccga
     2401 tgctgcgtgc ggaatacgat gaaaacccgg aagcgctggc gggtaccccg accgacccgg
     2461 tgcgtttcga tcgtgacgat ctgcgtgagc gtgaactgga gtatggcaag gcgggcttca
     2521 ccctgcaatt tatgctgaac ccgaacctga gcgacgcgga gaagtacccg ctgcgtctgc
     2581 gtgatgcgat tgtggcggcg ctggatctgg aaaaagcgcc gatgcactat cagtggctgc
     2641 cgaaccgtca aaacattatc gaggacctgc cgaacgttgg tctgaaaggc gacgatctgc
     2701 acacctatca cgattgcagc aacaacagcg gccagtacca gcaaaagatt ctggtgatcg
     2761 acccgagcgg tcgtggcaaa gatgaaaccg gttacgcggt tctgtatacc ctgaacggct
     2821 acatttatct gatggaggcg ggtggcttcc gtgacggcta cagcgataag accctggaac
     2881 tgctggcgaa gaaagcgaaa cagtggggtg tgcaaaccgt ggtttatgag agcaactttg
     2941 gtgacggcat gttcggcaag gtttttagcc cgatcctgct gaaacaccac aactgcgcga
     3001 tggaggaaat tcgtgcgcgt ggtatgaaag agatgcgtat ctgcgatacc ctggaaccgg
     3061 tgatgcagac ccaccgtctg gtgattcgtg acgaagttat ccgtgcggat taccaaagcg
     3121 cgcgtgacgt ggatggcaag cacgacgtta aatacagcct gttttatcag atgacccgta
     3181 ttacccgtga gaagggtgcg ctggcgcatg acgatcgtct ggatgcgctg gcgctgggca
     3241 tcgagtatct gcgtgaaagc atgcaactgg acagcgtgaa agttgaaggt gaggttctgg
     3301 cggatttcct ggaggaacac atgatgcgtc cgaccgtggc ggcgacccac attatcgaaa
     3361 tgagcgttgg tggcgtggac gtttacagcg aggacgatga aggttatggc accagcttta
     3421 tcgagtggta atgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
     3481 gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4321 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4741 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5221 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5281 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5341 ccgcagccga a
//
