LOCUS       sequence-369665-        5351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Large terminase from phage T3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_T3
FEATURES             Location/Qualifiers
     source          1..5351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        835..1119
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1042..1061
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2934..2961
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2980..3071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3072..3140,3141..3932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3290..3309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4103..4691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4592..4611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4845..4862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4877..5017
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4943..4962)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5103..5125
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(5281..5351,1..808))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
       61 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
      121 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
      181 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
      241 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
      301 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
      361 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
      421 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
      481 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
      541 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
      601 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
      661 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
      721 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
      781 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
      841 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
      901 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
      961 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     1021 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     1081 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt gggctaacag
     1141 gaggaattaa ccatgtctac tcaatccaat cgtaatgcgc tcgtagtggc gcaactgaaa
     1201 ggagacttcg tggcgttcct gttcgtctta tggaaggcgt taaatcttcc ggtgccaact
     1261 aagtgccaaa tagatatggc taaggtgctg gcgaatggag acaacaagaa gttcatccta
     1321 caggctttcc gtggtatcgg taagtcgttc atcacatgtg cgttcgttgt gtggacgtta
     1381 tggcgtgacc ctcagttgaa gatactgatt gtctctgcat ccaaagaacg tgcggacgct
     1441 aactccatct tcatcaagaa catcattgac ctgctgccct tcttggctga gctaaagcca
     1501 cgccccggtc agcgtgactc tgtgattagt ttcgatgttg gccctgccaa gcctgaccac
     1561 tctccgtctg tgaagtcggt gggtatcact ggtcagttga ctggtagccg tgctgatatc
     1621 atcatagcgg atgacgttga gattccgtct aactccgcaa ctcaaggtgc ccgtgagaag
     1681 ctgtggactc tggtgcagga atttgctgcg cttctgaaac cgctgccgac ctctcgcgtt
     1741 atctaccttg gtacgcctca aaccgaaatg accttgtaca aggaactcga agataaccgt
     1801 gggtacacca caatcatctg gcctgcgctc tatccgcgta gccgtgagga agacttgtac
     1861 tatggtgaac gtctggcccc tatgctccgt gaggagttca acgatgggtt cgagatgctc
     1921 caaggccaac caactgaccc cgtgcgattc gacatggaag acctacggga acgtgagttg
     1981 gaatacggta aggctggctt cactttacag ttcatgctta acccgaacct tagtgatgcc
     2041 gagaagtacc ccttacgcct ccgtgacgct atcgtgtgcg gtcttgactt cgagaaagcc
     2101 ccaatgcact accagtggct tccgaaccgt cagaatcgca atgaggagct gcctaacgtg
     2161 ggccttaagg gtgatgacat tcatagctat cattcgtgca gccagaacac tggacagtac
     2221 cagcaacgca tcctcgtgat tgaccctagt ggtcgaggta aggatgagac aggttacgca
     2281 gtgttgttca ccctgaatgg ctacatctat ctgatggaag ctggcgggtt ccgcgatggt
     2341 tattccgata agacccttga gtccctcgct aagaaagcga agcagtggaa ggttcagaca
     2401 gtggtcttcg agagtaactt cggggatggt atgtttggta aggtattcag ccctgtgctc
     2461 cttaaacacc atgcagcagc tctggaagag attcgtgctc gtggtatgaa ggaactacgc
     2521 atttgtgata cgctggagcc tgtactctct acgcaccgcc ttgtgattcg tgatgaggtg
     2581 attcgtgagg actaccagac tgcccgtgac gctgatggca agcatgacgt tcgctattcg
     2641 ctgttctacc agttgacccg tatggctcgt gagaagggcg ctgtggccca tgatgaccga
     2701 cttgatgcgt tagcgttagg tgtggagttc ttgcgctcta cgatggaact ggacgccgtg
     2761 aaggtggaag ccgaggtgct tgaggcattc ttagaggaac acatggagca cccaatacat
     2821 tcggctggtc acgtagttac cgctatggtt gacgggatgg aactctattg ggaggatgac
     2881 gatgtgaatg gcgataggtt cattaactgg taatgccagg catcaaatta agcagaaggc
     2941 catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac
     3001 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3061 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3121 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3181 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3241 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3301 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3361 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3421 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3481 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3541 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3601 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3661 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3721 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3781 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3841 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3901 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3961 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4021 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4081 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4141 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4201 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4261 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4321 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4381 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4441 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4501 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4561 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4621 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4681 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4741 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4801 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4861 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4921 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     4981 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     5041 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5101 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca
     5161 gatcaattcg cgcgcgaagg cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc
     5221 agggattctg caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa
     5281 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
     5341 cgggctggcc c
//
