LOCUS       sequence-369666-       11788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 11788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM763
FEATURES             Location/Qualifiers
     source          1..11788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     965..984
                     /label=LacI-R
                     /note="LacI, reverse primer"
     misc_RNA        complement(1407..1482)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(join(1642..3228,3229..3231,3232..5718,5719..5721,
                     5722..5748))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGEFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    5793..5809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5826..5842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      6775..7163
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7334..7443
                     /note="incP origin of transfer"
     CDS             complement(join(7574..8365,8366..8434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTSLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8197..8216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(join(11709..11788,1..1003))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
       61 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
      121 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcctcggtat
      181 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
      241 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
      301 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
      361 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
      421 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
      481 ccagatgctc cacgcccagt cgcgtaccgt cctcatggga gaaaataata ctgttgatgg
      541 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
      601 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
      661 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
      721 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
      781 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
      841 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
      901 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
      961 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc actagtgcca
     1021 tatgttaaaa acagatttgg agtgcaaagt tacgacaaat aatttgttaa catacatatt
     1081 taggcaactt tttcactata ttttcaacta ataaaacaaa aaagaatgcc gacaagttgt
     1141 gtgacggcat cctttttcta tttcattagc ttatcctaca atcttcttac cttaatttat
     1201 aatttattcc tgagtcagat caactgcaaa gtatccgcgt ttaccggatg ggaatacacc
     1261 tgtcaggaac aatacctgat tcggagactt aacagcagct ttcattgctt cttccagatc
     1321 aaggctattg ttgcagccaa tgcacaaatg ctgtgaaacg atcgtcaaat tgtacgcaag
     1381 atccttaatc atcatctaga aaaaaagcac cgactcggtg ccactttttc aagttgataa
     1441 cggactagcc ttattttaac ttgctatttc tagctctaaa actgcctgat gaggttttgt
     1501 ttactgcaaa gataagaact tttagcttta tcttccaaac tttatcggaa gtttttttgt
     1561 tcttatggtc aagcagtact gctgcagggt gctaggattt ttttgggcgg ccccgcccaa
     1621 aaaaattgca gccgcattaa ttcagtcacc tcctagctga ctcaaatcaa tgcgtgtttc
     1681 ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt ttgtagacgt
     1741 atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag ctccaagatt
     1801 cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt tgtctctatg
     1861 tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa cacgcttaga
     1921 aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct ccacaaacaa
     1981 ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac tagctaaata
     2041 taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta attctccggc
     2101 actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt taggtagttt
     2161 aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt caatcggatt
     2221 tttttcaaag gaacttcttt ccataattgt gatccctagt aactctttaa cggattttaa
     2281 cttcttcgat ttcccttttt ccaccttagc aaccactagg actgaataag ctaccgttgg
     2341 actatcaaaa ccaccatatt tttttggatc ccagtctttt ttacgagcaa taagcttgtc
     2401 cgaatttctt tttggtaaaa ttgactcctt ggagaattcg cctgtctgta cttctgtttt
     2461 cttgacaata ttgacttggg gcatggacaa tactttgcgc actgtggcaa aatctcgccc
     2521 tttatcccag acaatttctc cagtttcccc attagtttcg attagagggc gtttgcgaat
     2581 ctctccattt gcaagtgtaa tttctgtttt gaagaagttc atgatattag agtaaaagaa
     2641 atattttgcg gttgctttgc ctatttcttg ctcagactta gcaatcattt tacgaacatc
     2701 ataaacttta taatcaccat agacaaactc cgattcaagt tttggatatt tcttaatcaa
     2761 agcagttcca acgacggcat ttagatacgc atcatgggca tgatggtaat tgttaatctc
     2821 acgtacttta tagaattgga aatcttttcg gaagtcagaa actaatttag attttaaggt
     2881 aatcacttta acctctcgaa taagtttatc attttcatcg tatttagtat tcatgcgact
     2941 atccaaaatt tgtgccacat gcttagtgat ttggcgagtt tcaaccaatt ggcgtttgat
     3001 aaaaccagct ttatcaagtt cactcaaacc accacgttca gctttcgtta aattatcaaa
     3061 cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt tcatcttttt
     3121 gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag aacgcgttaa
     3181 gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgg catcgacatc
     3241 ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc ttccattttg
     3301 gagataatag agatagagct tttcattttg caattgagta ttttcaacag gatgctcttt
     3361 aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac gctctcgcga
     3421 atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa cgatattttc
     3481 tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag tctgtaaaat
     3541 acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta aactatcgcc
     3601 ttgtccagac acttgtgctt tttgaatgtc ttctttaaat gtcaaactat catcatggat
     3661 cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta atattgtttt
     3721 gccagattgc ttatccctaa taccattaat caattttcga gacaaacgtc cccaaccagt
     3781 ataacggcga cgtttaagct gtttcatcac cttatcatca aagaggtgag catatgtttt
     3841 aagtctttcc tcaatcatct ccctatcttc aaataaggtc aatgttaaaa caatatcctc
     3901 taagatatct tcattttctt cattatccaa aaaatcttta tctttaataa tttttagcaa
     3961 atcatggtag gtacctaatg aagcattaaa tctatcttca actcctgaaa tttcaacact
     4021 atcaaaacat tctatttttt tgaaataatc ttcttttaat tgcttaacgg ttacttttcg
     4081 atttgttttg aagagtaaat caacaatggc tttcttctgt tcacctgaaa gaaatgctgg
     4141 ttttcgcatt ccttcagtaa catatttgac ctttgtcaat tcgttataaa ccgtaaaata
     4201 ctcataaagc aaactatgtt ttggtagtac tttttcattt ggaagatttt tatcaaagtt
     4261 tgtcatgcgt tcaataaatg attgagctga agcaccttta tcgacaactt cttcaaaatt
     4321 ccatggggta attgtttctt cagacttccg agtcatccat gcaaaacgac tattgccacg
     4381 cgccaatgga ccaacataat aaggaattcg aaaagtcaag attttttcaa tcttctcacg
     4441 attgtctttt aaaaatggat aaaagtcttc ttgtcttctc aaaatagcat gcagctcacc
     4501 caagtgaatt tgatggggaa tagagccgtt gtcaaaggtc cgttgcttgc gcagcaaatc
     4561 ttcacgattt agtttcacca ataattcctc agtaccatcc attttttcta aaattggttt
     4621 gataaattta taaaattctt cttggctagc tcccccatca atataacctg catatccgtt
     4681 ttttgattga tcaaaaaaga tttctttata cttttctgga agttgttgtc gaactaaagc
     4741 ttttaaaaga gtcaagtctt gatgatgttc atcgtagcgt ttaatcattg aagctgatag
     4801 gggagcctta gttatttcag tatttactct taggatatct gaaagtaaaa tagcatctga
     4861 taaattctta gctgccaaaa acaaatcagc atattgatct ccaatttgcg ccaataaatt
     4921 atctaaatca tcatcgtaag tatcttttga aagctgtaat ttagcatctt ctgccaaatc
     4981 aaaatttgat ttaaaattag gggtcaaacc caatgacaaa gcaatgagat tcccaaataa
     5041 gccatttttc ttctcaccgg ggagctgagc aatgagattt tctaatcgtc ttgatttact
     5101 caatcgtgca gaaagaatcg ctttagcatc tactccactt gcgttaatag ggttttcttc
     5161 aaataattga ttgtaggttt gtaccaactg gataaatagt ttgtccacat cactattatc
     5221 aggatttaaa tctccctcaa tcaaaaaatg accacgaaac ttaatcatat gcgctaaggc
     5281 caaatagatt aagcgcaaat ccgctttatc agtagaatct accaattttt ttcgcagatg
     5341 atagatagtt ggatatttct catgataagc aacttcatct actatatttc caaaaatagg
     5401 atgacgttca tgcttcttgt cttcttccac caaaaaagac tcttcaagtc gatgaaagaa
     5461 actatcatct actttcgcca tctcatttga aaaaatctcc tgtagataac aaatacgatt
     5521 cttccgacgt gtataccttc tacgagctgt ccgtttgaga cgagtcgctt ccgctgtctc
     5581 tccactgtca aataaaagag cccctataag attttttttg atactgtggc ggtctgtatt
     5641 tcccagaacc ttgaactttt tagacggaac cttatattca tcagtgatca ccgcccatcc
     5701 gacgctattt gtgccgatag ctaagcctat tgagtatttc ttatccatgt ttatattatt
     5761 tatatttgtt tgacgagaat atctttattt aattgttatc cgctcacaat tgccgacaaa
     5821 ggtaattgtt atccgctcac aattcaaata aatagataga aaaataacag tttgtcgaat
     5881 tttctttgta accaccgacg ctaagatgct ggccacgtgc taaggagtga gcttctcgga
     5941 ttttatttgt atttttgcca tgcctgatga ggttttgttt gattattttt ttgcaacact
     6001 aagttaagtg aatcctctga catggcaaaa tcctgagcaa ctttttgttg ctcaggtact
     6061 taaaaaaaat attttataat agtgttgcgg aattaaggta aaagaataaa atggttttta
     6121 ctctggaaga ttttgttggc gattggcgtc agaccgcggg ttataatttg gatcaagtcc
     6181 tggaacaggg tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg acgccgattc
     6241 agcgcatcgt tctgtccggc gagaacggtc tgaaaattga tattcatgtg atcatcccgt
     6301 acgaaggcct gagcggtgac caaatgggtc aaatcgagaa aatctttaaa gtcgtctacc
     6361 cagttgacga tcaccacttc aaggttatct tgcattacgg tacgctggtg attgatggtg
     6421 tgaccccgaa tatgattgac tatttcggcc gtccgtatga aggcattgcc gtttttgacg
     6481 gtaaaaagat caccgtcacc ggtaccctgt ggaatggcaa taagattatt gacgagcgtc
     6541 tgattaaccc ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc acgggttggc
     6601 gtctgtgcga gcgcatcctg gcataatgaa ctgcacttgc tttgataatt aatgataaac
     6661 aatctaaaag cactctaatc gttatcggag tgcttttaga ttactaatca aattgcttct
     6721 actaattgcc tatcttccag tgatggaacc caccgcggtg gaggggaatt cccatgtcag
     6781 ccgttaagtg ttcctgtgtc actcaaaatt gctttgagag gctctaaggg cttctcagtg
     6841 cgttacatcc ctggcttgtt gtccacaacc gttaaacctt aaaagcttta aaagccttat
     6901 atattctttt ttttcttata aaacttaaaa ccttagaggc tatttaagtt gctgatttat
     6961 attaatttta ttgttcaaac atgagagctt agtacgtgaa acatgagagc ttagtacgtt
     7021 agccatgaga gcttagtacg ttagccatga gggtttagtt cgttaaacat gagagcttag
     7081 tacgttaaac atgagagctt agtacgtgaa acatgagagc ttagtacgta ctatcaacag
     7141 gttgaactgc tgatcttcag atcctctacg ccggacgcat cgtggccgga tcaattccgt
     7201 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
     7261 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
     7321 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     7381 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     7441 cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca
     7501 ggaagggcag cccacctatc acggaattga tccccctcga attgacgcgt tatgagtaaa
     7561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7681 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7921 gtatggcttc attcagctcc ggttcccaac gatcaaggct agttacatga tcccccatgt
     7981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8281 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8341 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8401 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatggccgc
     8461 gggattaaaa gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag agaaatatcg
     8521 ttttgctaaa cagttgatat tgaggtatca ttttatcgta aaagacattt ttgctcaaca
     8581 attgcttgac ggaaatcaac aaattttagc attttgtaaa aaagtcgcta tataatttgg
     8641 tgaattggag ttattttcat atttttgcat cccgaagagt ttctcttaaa gagagaaaca
     8701 tcttttgcat accttttccg accgaatttt tatgtcgtaa agaggggctt tgcagggggt
     8761 ggactcagaa agatgagaat agatgactat tgtagttgaa acacatagaa agttgctgat
     8821 atacagaccg atacgcatat cgggatgaac catgagtacg ttcttttctc aaaaaacata
     8881 aatattcgaa aagagatgca ataaattaag gagaggttat aatgaacaaa gtaaatataa
     8941 aagatagtca aaattttatt acttcaaaat atcacataga aaaaataatg aattgcataa
     9001 gtttagatga aaaagataac atctttgaaa taggtgcagg gaaaggtcat tttactgctg
     9061 gattggtaaa gagatgtaat tttgtaacgg cgatagaaat tgattctaaa ttatgtgagg
     9121 taactcgtaa taagctctta aattatccta actatcaaat agtaaatgat gatatactga
     9181 aatttacatt tcctagccac aatccatata aaatatttgg cagcatacct tacaacataa
     9241 gcacaaatat aattcgaaaa attgtttttg aaagttcagc cacaataagt tatttaatag
     9301 tggaatatgg ttttgctaaa atgttattag atacaaacag atcactagca ttgctgttaa
     9361 tggcagaggt agatatttct atattagcaa aaattcctag gtattatttc catccaaaac
     9421 ctaaagtgga tagcacatta attgtattaa aaagaaagcc agcaaaaatg gcatttaaag
     9481 agagaaaaaa atatgaaact tttgtaatga aatgggttaa caaagagtac gaaaaactgt
     9541 ttacaaaaaa tcaatttaat aaagctttaa aacatgcgag aatatatgat ataaacaata
     9601 ttagtttcga acaatttgta tcgctattta atagttataa aatatttaac ggctaaaaac
     9661 aataggccac atgcaactgt aaatgtttac gcgtcctcgg taccaacaaa tactttcagg
     9721 acgatgtaaa gtcctgagaa atcaaactga atggatagat aaatatttgt cttttctcat
     9781 cggattcttt taatatcgct actctctacg ttcaaccaaa agaaataatg acttactgct
     9841 atattttttg cacgtgtggg gaaaatgtgg ggaaaattca agcaaaagaa aaagctaagt
     9901 attgaactat caaatactta gctttctttc ttgtacccag accccgcatt tgaaataatt
     9961 aaagtgggga aaatgtgggt aaaaagaaaa atgcggaaaa acgccacaat tacactgtat
    10021 ttcaatatgt tataatccta ttaaatttta atccaagttt aatcgaattg caaaatattt
    10081 agcagatgtg gggaaaatgc tggggaaaat atttatattt gcagcagagt aaaattcaac
    10141 ctgaaagtct atgaaagtaa cctttatcat taaaaaagca gccaaacgat atgatacaga
    10201 atccatggct acaatctatg tccgttttag aaacggaagg cagttagact ccgttgctcc
    10261 tactcagtta gccatcaatc ccaatctatg ggatgataaa gacgaatgtg taaaaacgaa
    10321 agctgtctgc aatgaagaaa tgcgtaccca tataaatgaa gagatacgcc agttgaaaac
    10381 ctatatcgag aaggtatatc aacaagaaaa ggaagcaata gacaaagaat ggctaaaaac
    10441 aacacttgat aaattttacc atcctgaaaa atattttttg ccggaggaag tggttatcaa
    10501 gcctaccatt ggagaactat tcgatgaatt tctaaacaag caccctttgt cggaagtacg
    10561 aaagaaaaat ttccgggttg tcaaaagagc cttactgcgt tatgaactat atgtaagggc
    10621 tacaaagaga ggacaaaagg gctttatcct tgatgtggat ttggtaacac ctgacacgct
    10681 tcgggatatg tgggatttct ttcagaacga ataccagtat tatgaacttt acccgagcat
    10741 ttatgaagcc attcccgaaa agaggacacc acagcccaga agcaaaaaca cgctgataga
    10801 ctgtttttca agaatacgca cattcttcct gtggtgcttc gataacaaac gcaccacaaa
    10861 cagacctttc gacaagtttc cgatagagga gtgtacatat ggtacacctt attatataac
    10921 actcgaagaa agggacagga tttttaatgc agacctttct gccaccccac aactggcaat
    10981 acagagggat atattcatat ttcagacact gataggatgc agggtgagcg acctgtaccg
    11041 aatgaccaaa ctaaatgtgg tcaatgaagc catagaatat attcccaaga aaaccaaaga
    11101 ggggaatccg gttacggtac gtgttccact taacgacaaa gcgaaagaaa tccttgaacg
    11161 ctacaaagaa tatgagggaa aactgttgcc gttcatatcc gagcaaaagt acaatgatgc
    11221 cataaaaaag atattcaaat tagctggagt tgaccgcatc gtaacaatct tagacccgtt
    11281 gacgcacaac gaaatcaaac gacctattta tgaagtggca agcagccatc tggcaagacg
    11341 tacgtttatc ggcaatatct ataaaaaagt gaaagacccg aaccttgttt ccgcactgtc
    11401 gggacacaag gagggaagca aagctttcag acgatacagg gatattgacg aagaaatgaa
    11461 gaaagacctt gtaaaactac tggactgata aaagtatcaa acagtttcat agatagttac
    11521 caatgactat atttgtagaa attatcatca caatatgagc aacaaggaat ccaacgtaga
    11581 gaaaacgaat tttgatgcat tcatacaagc ggtcggttcg gaaatagaac aggcacaagt
    11641 aggaccaaaa cgaaaaaagg cccccctttc gggaggcctc ttttctggaa tttggtaccg
    11701 aggaaagctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    11761 tcggccaacg cgcggggaga ggcggttt
//
