LOCUS       sequence-369667-        5408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from human herpesvirus 8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_HHV8
FEATURES             Location/Qualifiers
     source          1..5408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..57
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    72..212
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(138..157)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     298..320
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(476..1354)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1381..1665
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1588..1607
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3537..3564
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3583..3674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3675..3743,3744..4535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3893..3912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4706..5294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5195..5214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
       61 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      121 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
      181 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
      241 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
      301 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca
      361 attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac gaagcaggga
      421 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg
      481 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
      541 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
      601 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
      661 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
      721 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
      781 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat
      841 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
      901 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
      961 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg
     1021 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
     1081 accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca
     1141 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc
     1201 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
     1261 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
     1321 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
     1381 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
     1441 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
     1501 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
     1561 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
     1621 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
     1681 taacaggagg aattaaccat gctgcgtatg aacccgggtc tgggcagcag catcagcgtt
     1741 cacccgagcg aactgagcat cagcctgttc gagattctgc agggcaagta cagctatgtt
     1801 cgtggtcaga ccctgcactg cagcctgcgt aacccgggcg tgttctttcg tcaactgttt
     1861 attcacctgt acaaaaacgc gctggcgaac tgcagctatg atcacgtgct gagcgactgg
     1921 cgtacctacg agagcagcgc gaagacccgt tggccggaga aagaagcgca atggggtagc
     1981 tatcgtcgta gcaccttcga tagctgggcg cagaccatgc gtatgaccct ggaccacctg
     2041 ctgctgaacg cgatcaaccg tgttctgtac gcgaagaccc agctgagcta cgaacgttat
     2101 gtggattggg tggttaccgt tggtatggtg ccggtggtta agcacacccc ggatcacaaa
     2161 ctggttaaca gcatccagga acaactgatg aaggactgcc aacgtctggc gagcggcgag
     2221 aaaaccatcg gtcgtattct gaccagcgtt acccaggaaa ttagcaacct ggttagcagc
     2281 ctgagcgcgc tgtacatccc gggctatagc gaggtttcta ttgactacga ttgcgttaaa
     2341 aacaccttcg tgggtctgta taagcagaaa cgtgtgcacg ttgaagtgat caccatgccg
     2401 gcgattctgg cgggccgtgt gatctttgac agcccgattc aacgtatgta caccagcatc
     2461 atgagctgcc accgtaccgc ggagcatgcg aagctgtgcc agctgctgaa caccgcgccg
     2521 accaaagcgc tggttggtag cgcgtgcaac aacgtgtata aggatattat gacccacctg
     2581 gaacaggcga gccaacgtac cgacccgaaa cgtgaactgc tgaacctgct gatgaagctg
     2641 gcggagaaca aaaccgttag cggcgtgacc gacgtggttg aggatttcgt taccgacgtg
     2701 agccagaaca tcgttgataa gaacaaactg tttggtaccg gccaggaaac caccacccaa
     2761 ggtctgcgtc gtcaagttag caacagcgtg ttcaagtgcc tgaccaacca gatcaacgag
     2821 caatttgaca ccattaccca actggagaaa gaacgtgagc tgtgcatgaa gcgtctgaaa
     2881 tgcattgaaa cccagctgag ccaccagcaa ccgggtgatg cgaaaggtcc gggcagcgtg
     2941 aacctgctga ccgcgaacac cttccaaagc ctgggccgtc tgcaagaccc gagcctgcag
     3001 ctgaccagca gccacatccc gagcggtagc gcggttctga acagcttctt tagcagctac
     3061 attccgccgg tgcgtgagag catgaaggat ctgaccaacc tgtgggaaag cgagatgttt
     3121 cagacctata aactggcgcc ggtggttgat aaccagggcc aacgtctgag cgttacctac
     3181 agccaagaca ccatcagcat tctgctgggt ccgttcacct atgttatcgc ggacctgctg
     3241 cagatggaac tgattagcca cagctttgtg agcagcagcc tgcaagacat cgcggcgtac
     3301 ctgtatcaga ccagccgtct gttcgtttac attaccgacg tgggccagaa gtattgcctg
     3361 gttaccccgc cgtttgaaaa cgtgccgggt aaaggcccgg gcgagaccga ttggagcgcg
     3421 aacgaatata gctgcccgga ggacagccgt gttcgtcgtg gtctgagccg tatcccgccg
     3481 ccgtgcggcg ctccgccgtg cccgggtagc gcgtaatgcc aggcatcaaa ttaagcagaa
     3541 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa
     3601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3901 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5401 cctttgag
//
