LOCUS       sequence-369668-        5165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage T4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_T4
FEATURES             Location/Qualifiers
     source          1..5165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..64
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    79..219
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(145..164)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     305..327
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(483..1361)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1388..1672
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1595..1614
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3301..3328
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3347..3438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3439..3507,3508..4299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3657..3676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4470..5058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4959..4978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
       61 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      121 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
      181 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
      241 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      301 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
      361 cagatcaatt cgcgcgcgaa ggcgaagcgg catgcataat gtgcctgtca aatggacgaa
      421 gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc
      481 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
      541 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
      601 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
      661 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
      721 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
      781 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc
      841 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga
      901 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca
      961 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca
     1021 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac
     1081 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg
     1141 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc
     1201 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa
     1261 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac
     1321 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca
     1381 ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac
     1441 tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc
     1501 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
     1561 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
     1621 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt
     1681 tttgggctaa caggaggaat taaccatgaa atttaatgta ttaagtttgt ttgctccatg
     1741 ggctaaaatg gacgaacgaa attttaaaga ccaagaaaaa gaagatcttg tttccattac
     1801 agccccaaag cttgatgatg gagcaagaga atttgaagta agctcgaatg aagctgcttc
     1861 tccttataat gctgcattcc aaacaatttt tggttcatat gaaccaggaa tgaaaactac
     1921 tcgtgagctt attgatacat atcgtaatct catgaataac tatgaagtag ataatgcagt
     1981 ttcagaaatc gtttcagatg ctatcgtcta tgaagatgat actgaagtcg tagcgttaaa
     2041 tttggataaa tctaaattta gcccaaaaat taaaaatatg atgttagatg aatttagtga
     2101 tgtattaaat catctatcgt ttcaacgaaa aggttctgat cattttagac gttggtatgt
     2161 tgattcaaga attttctttc ataaaatcat tgatccaaaa cgtccaaaag aaggcataaa
     2221 agaattacgt agattagacc ctcgccaagt tcagtatgtt cgtgaaatta taacagaaac
     2281 tgaagctggc acaaaaatag ttaaaggtta caaagaatat tttatatatg atactgccca
     2341 tgagtcatat gcatgtgatg gtagaatgta tgaagctggc acaaaaataa aaattcctaa
     2401 agctgccgtc gtttatgccc attctggatt agtcgattgt tgcggtaaaa atatcatcgg
     2461 gtatttgcat cgtgctgtta aacctgctaa ccaattaaaa ttattagaag atgctgtagt
     2521 catttatcgc attactcgtg ctcctgaccg tcgtgtttgg tatgtagaca caggtaatat
     2581 gcctgctcgt aaagctgctg agcacatgca acatgttatg aacacgatga aaaaccgtgt
     2641 agtatatgat gcatcaacag gtaaaataaa aaatcaacag cataatatgt ctatgaccga
     2701 agactattgg ttgcagcgcc gtgatggtaa agctgtgaca gaagttgata ctcttcctgg
     2761 tgctgataat actggcaata tggaagatat tcgttggttt agacaagctc tttatatggc
     2821 attacgtgtt cctctttcac gcattccgca agaccaacaa ggcggtgtga tgtttgattc
     2881 tggaactagc attacacgtg atgaattaac gtttgctaaa tttattcgtg agttacagca
     2941 caagtttgaa gaagttttcc tagatccgct taaaacaaat cttttgctta aaggtataat
     3001 cacagaagat gagtggaatg atgaaataaa taatattaag atagaatttc atcgggatag
     3061 ctactttgct gagctcaaag aagcagaaat tttggaacga agaattaata tgctaaccat
     3121 ggcagaacca tttattggta aatatatttc tcacagaact gctatgaaag acattttgca
     3181 gatgactgat gaagaaatag aacaagaagc caagcaaatt gaagaagagt ctaaagaggc
     3241 tcgtttccaa gaccccgacc aagaacaaga ggatttttaa tgccaggcat caaattaagc
     3301 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
     3361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3661 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     3721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3961 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5161 accgc
//
