LOCUS       sequence-369669-       10131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Guo LY, Bian J, Davis AE, Liu P, Kempton HR, Zhang X, Chemparathy A,
            Gu B, Lin X, Rane DA, Xu X, Jamiolkowski RM, Hu Y, Wang S, Qi LS
  TITLE     Multiplexed genome regulation in vivo with hyper-efficient Cas12a.
  JOURNAL   Nat Cell Biol. 2022 Apr;24(4):590-600. doi: 
            10.1038/s41556-022-00870-7. Epub 2022 Apr 12.
  PUBMED    35414015
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00870-7"; journalName: "Nat Cell Biol"; date: 
            "2022-04"; volume: "24"; issue: "4"; pages: "590-600"
COMMENT     Sequence Label: pSLQ10873
FEATURES             Location/Qualifiers
     source          1..10131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1235..4918
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4925..4951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4931..4957
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4970..4996
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5006..5032
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5069..5218
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5225..5245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5378..5734
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     primer_bind     complement(6046..6065)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6111..6166
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6165..6184)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6527..6543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6540..6562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6551..6567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6575..6581,6582..6599,6600..6605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6620..6641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6700..6895
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6746..6881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6901..7035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6938..6957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6992..7011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7103..7120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7274..7862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7354..7373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8033..8824,8825..8893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8656..8675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8894..8998)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9054..9302
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9054..9074
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9225..9244
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        9612..9991
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg
     1201 acggtaccgc gggcccggga tccaccgggc caccatgagc aagctggaga agtttacaaa
     1261 ctgctactcc ctgtctaaga ccctgaggtt caaggccatc cctgtgggca agacccagga
     1321 gaacatcgac aataagcggc tgctggtgga ggacgagaag agagccgagg attataaggg
     1381 cgtgaagaag ctgctggatc gctactatct gtcttttatc aacgacgtgc tgcacagcat
     1441 caagctgaag aatctgaaca attacatcag cctgttccgg aagaaaacca gaaccgagaa
     1501 ggagaataag gagctggaga acctggagat caatctgcgg aaggagatcg ccaaggcctt
     1561 caagggcaac gagggctaca agtccctgtt taagaaggat atcatcgaga caatcctgcc
     1621 agagttcctg gacgataagg acgagatcgc cctggtgaac agcttcaatg gctttaccac
     1681 agccttcacc ggcttctttc gtaacagaga gaatatgttt tccgaggagg ccaagagcac
     1741 atccatcgcc ttcaggtgta tcaacgagaa tctgacccgc tacatctcta atatggacat
     1801 cttcgagaag gtggacgcca tctttgataa gcacgaggtg caggagatca aggagaagat
     1861 cctgaacagc gactatgatg tggaggattt ctttgagggc gagttcttta actttgtgct
     1921 gacacaggag ggcatccgcg tgtataacgc catcatcggc ggcttcgtga ccgagagcgg
     1981 cgagaagatc aagggcctga acgagtacat caacctgtat aatcagaaaa ccaagcagaa
     2041 gctgcctaag tttaagccac tgtataagca ggtgctgagc gatcgggagt ctctgagctt
     2101 ctacggcagg ggctatacat ccgatgagga ggtgctggag gtgtttagaa acaccctgaa
     2161 caagaacagc gagatcttca gctccatcaa gaagctggag aagctgttca agaattttga
     2221 cgagtactct agcgccggca tctttgtgaa gaacggcccc gccatcagca caatctccaa
     2281 gcgtatcttc ggcgagtgga acgtgatccg ggacaagtgg aatgccgagt atgacgatat
     2341 ccacctgaag aagaaggccg tggtgaccga gaagtacgag gacgatcgga gaaagtcctt
     2401 caagaagatc ggctcctttt ctctggagca gctgcaggag tacgccgacg ccgatctgtc
     2461 tgtggtggag aagctgaagg agatcatcat ccagaaggtg gatgagatct acaaggtgta
     2521 tggatcctct gagaagctgt tcgacgccga ttttgtgctg gagaagagcc tgaagaagaa
     2581 cgacgccgtg gtggccatca tgaaggacct gctggattct gtgaagagct tcgagaatta
     2641 catcaaggcc ttctttggcg agggcaagga gacaaacagg gacgagtcct tctatggcga
     2701 ttttgtgctg gcctacgaca tcctgctgaa ggtggaccac atctacgatg ccatccgcaa
     2761 ttatgtgacc cagaagccct actctaagga taagttcaag ctgtattttc agaaccctca
     2821 gttcatgggc ggctgggaca aggataagga gacagactat cgggccacca tcctgagata
     2881 cggctccaag tactatctgg ccatcatgga taagaagtac gccaagtgcc tgcagaagat
     2941 cgacaaggac gatgtgaacg gcaattacga gaagatcaac tataagctgc tgcccggccc
     3001 taataagatg ctgccaaagg tgttcttttc taagaagtgg atggcctact ataaccccag
     3061 cgaggacatc cagaagatct acaagaatgg cacattcaag aagggcgata tgtttaacct
     3121 gaatgactgt cacaagctga tcgacttctt taaggatagc atctcccggt atccaaagtg
     3181 gtccaatgcc tacgatttca acttttctga gacagagaag tataaggaca tcgccggctt
     3241 ttacagagag gtggaggagc agggctataa ggtgagcttc gagtctgcca gcaagaagga
     3301 ggtggataag ctggtggagg agggcaagct gtatatgttc cagatctata acaaggactt
     3361 ttccgataag tctcacggca cacccaatct gcacaccatg tacttcaagc tgctgtttga
     3421 cgagaacaat cacggacaga tcaggctgag cggaggagca gagctgttca tgaggcgcgc
     3481 ctccctgaag aaggaggagc tggtggtgca cccagccaac tcccctatcg ccaacaagaa
     3541 tccagataat cccaagaaaa ccacaaccct gtcctacgac gtgtataagg ataagaggtt
     3601 ttctgaggac cagtacgagc tgcacatccc aatcgccatc aataagtgcc ccaagaacat
     3661 cttcaagatc aatacagagg tgcgcgtgct gctgaagcac gacgataacc cctatgtgat
     3721 cggcatcgcc aggggcgagc gcaatctgct gtatatcgtg gtggtggacg gcaagggcaa
     3781 catcgtggag cagtattccc tgaacgagat catcaacaac ttcaacggca tcaggatcaa
     3841 gacagattac cactctctgc tggacaagaa ggagaaggag aggttcgagg cccgccagaa
     3901 ctggacctcc atcgagaata tcaaggagct gaaggccggc tatatctctc aggtggtgca
     3961 caagatctgc gagctggtgg agaagtacga tgccgtgatc gccctggagg acctgaactc
     4021 tggctttaag aatagccgcg tgaaggtgga gaagcaggtg tatcagaagt tcgagaagat
     4081 gctgatcgat aagctgaact acatggtgga caagaagtct aatccttgtg caacaggcgg
     4141 cgccctgaag ggctatcaga tcaccaataa gttcgagagc tttaagtcca tgtctaccca
     4201 gaacggcttc atcttttaca tccctgcctg gctgacatcc aagatcgatc catctaccgg
     4261 ctttgtgaac ctgctgaaaa ccaagtatac cagcatcgcc gattccaaga agttcatcag
     4321 ctcctttgac aggatcatgt acgtgcccga ggaggatctg ttcgagtttg ccctggacta
     4381 taagaacttc tctcgcacag acgccgatta catcaagaag tggaagctgt actcctacgg
     4441 caaccggatc agaatcttcc ggaatcctaa gaagaacaac gtgttcgact gggaggaggt
     4501 gtgcctgacc agcgcctata aggagctgtt caacaagtac ggcatcaatt atcagcaggg
     4561 cgatatcaga gccctgctgt gcgagcagtc cgacaaggcc ttctactcta gctttatggc
     4621 cctgatgagc ctgatgctgc agatgcggaa cagcatcaca ggccgcaccg acgtggattt
     4681 tctgatcagc cctgtgaaga actccgacgg catcttctac gatagccgga actatgaggc
     4741 ccaggagaat gccatcctgc caaagaacgc cgacgccaat ggcgcctata acatcgccag
     4801 aaaggtgctg tgggccatcg gccagttcaa gaaggccgag gacgagaagc tggataaggt
     4861 gaagatcgcc atctctaaca aggagtggct ggagtacgcc cagaccagcg tgaagcacgg
     4921 atcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc ctgctgctaa
     4981 acgtgttaag cttgatgggg gtagcccggc agccaagaga gtcaaactcg acgacggcat
     5041 tggtagtggg agcaacggca gcagcctcga tgctttagac gattttgact tagatatgct
     5101 tggttcagac gcgttagacg acttcgacct agacatgtta ggctcagatg cattggacga
     5161 cttcgattta gatatgttgg gctccgatgc cctagatgac tttgatctag atatgctagg
     5221 tagtcccaaa aagaagagga aagtgggatc cgtccttccg caagcgcctg ccccagcgcc
     5281 tgcaccggca atggtgtccg ccctcgcaca ggcccctgcg cccgtccccg tgctcgcgcc
     5341 tggaccgccc caggcggtcg ctccaccggc tccgaagccg acgcaggccg gagagggaac
     5401 actctccgaa gcacttcttc aactccagtt tgatgacgag gatcttggag cactccttgg
     5461 aaactcgaca gaccctgcgg tgtttaccga cctcgcgtca gtagataact ccgaatttca
     5521 gcagcttttg aaccagggta tcccggtcgc gccacataca acggagccca tgttgatgga
     5581 ataccccgaa gcaatcacga gacttgtgac gggagcgcag cggcctcccg atcccgcacc
     5641 cgcacctttg ggggcacctg gcctccctaa cggacttttg agcggcgacg aggatttctc
     5701 ctccatcgcc gatatggatt tctcagcctt gctgtcaggc tctggcagcg gcagcgacct
     5761 ttcccatccg cccccaaggg gccatctgga tgagctgaca accacacttg agtccatgac
     5821 cgaggatctg aacctggact cacccctgac cccggaattg aacgagattc tggatacctt
     5881 cctgaacgac gagtgcctct tgcatgccat gcatatcagc acaggactgt ccatcttcga
     5941 cacatctctg ttttagtgta caggccgcac tcctcaggtg caggctgcct atcagaaggt
     6001 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     6061 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     6121 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     6181 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     6241 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     6301 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     6361 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     6421 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     6481 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     6541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6661 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
     6721 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6901 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6961 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7021 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7681 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9001 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagcaa ttggagggcc
     9061 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
     9121 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     9181 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     9241 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     9301 ccgaatttct actaagtgta gatttccctg acagccccca tcacataatt tctactaagt
     9361 gtagatatag caggcgcgga accccttcta atttctacta agtgtagata gcaacaggtc
     9421 acggcgcacg aatttctact aagtgtagat gctaccatgg caacgcgcag tggaatttct
     9481 actaagtgta gataacaagt taatagacaa ccatccaatt tctactaagt gtagattccc
     9541 acccccacag ctctgctcca atttctacta agtgtagatt tttttactcg agggatccat
     9601 taggcggccg cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     9661 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     9721 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     9781 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     9841 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     9901 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     9961 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
    10021 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
    10081 ttgtgcagcg atgggggcgg gggggggggc gcgcgccagg cggggcgggg c
//
