LOCUS       sequence-36967-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Zuniga JE, Schmidt JJ, Fenn T, Burnett JC, Arac D, Gussio R, 
            Stafford RG, Badie SS, Bavari S, Brunger AT
  TITLE     A potent peptidomimetic inhibitor of botulinum neurotoxin serotype A
            has a very different conformation than SNAP-25 substrate.
  JOURNAL   Structure. 2008 Oct 8. 16(10):1588-97.
  PUBMED    18940613
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2008 Oct 8. 16(10):1588-97."
COMMENT     Sequence Label: BoNT/A-LC
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(201..305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     373..391
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(415..429,430..435,436..452,453..459)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    435..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    467..483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gcctcattgg naacgttctt cggggcgaaa ctctcaagga tcttaccgct gttgagatcc
       61 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
      121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
      181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
      241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
      301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
      361 ttaacctata aaaataggcg tatcacgagg cccttttgtc ttcacctcga gaaatcataa
      421 aaaatttatt tgctttgtga gcggataaca attataatag attcaattgt gagcggataa
      481 caatttcaca cagaattcgc aattaaggag ataataggta tgccatttgt taataaacaa
      541 tttaattata aagatcctgt aaatggtgtt gatattgctt atataaaaat tccaaatgca
      601 ggacaaatgc aaccagtaaa agcttttaaa attcataata aaatatgggt tattccagaa
      661 agagatacat ttacaaatcc tgaagaagga gatttaaatc caccaccaga agcaaaacaa
      721 gttccagttt catattatga ttcaacatat ttaagtacag ataatgaaaa agataattat
      781 ttaaagggag ttacaaaatt atttgagaga atttattcaa ctgatcttgg gaagaatgtt
      841 gttaacatca atagtaaggg gaataccatt ttggggtgga agtacaatag atacagaatt
      901 aaaagttatt gatactaatt gtataatgtg atacaccngn gtgttancga atacagaagc
//
