LOCUS       sequence-369678-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-CHMP4C(121-233, M165C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: GB1-Delta-N-CHMP4C(M165C)
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             190..201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catatgcagt ataaactggc gctgaacggc aaaaccctga aaggcgaaac caccaccgaa
       61 gcggtggatg cggcgaccgc ggaaaaagtg tttaaacagt atgcgaacga taacggcgtg
      121 gatggcgaat ggacctatga tgatgcgacc aaaaccttta ccgtgaccga actggtgccg
      181 cgcggcagca ttgaaggccg cattcgcgaa aacctgaaag atagcggcct gtttctggaa
      241 catcatcatc atcatcatga aaacctgtat tttcaaggca gcgaaaacat ggatctgaac
      301 aaaattgatg atctgatgca agaaattacc gaacagcaag atattgcgca agaaattagc
      361 gaagcgttta gtcagcgcgt gggctttggc gatgattttg atgaagatga actgtgcgcg
      421 gaactggaag aactggaaca agaagaactg aacaaaaaaa tgaccaatat tcgcctgccg
      481 aacgttccga gcagcagcct gccggctcag ccgaaccgca aaccgggcat gagcagcact
      541 gcgcgtcgct cgcgggcggc gagcagtcag cgcgcggaag aagaagatga tgatattaaa
      601 cagctggcgg cgtgggcgac ctaaggatcc
//
