LOCUS       sequence-369682-        2007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing Terminator sequence (Bba_B0015).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_007
FEATURES             Location/Qualifiers
     source          1..2007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(111..902,903..971))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     734..753
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(972..1076)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1179..1767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1668..1687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1890..1961
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1977..2004
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ataaacgtga gaccaagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
       61 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      121 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      181 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      241 atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      301 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      361 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      421 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      481 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      541 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      601 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      661 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      721 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      781 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      841 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      901 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      961 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1021 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1081 tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1141 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1201 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1261 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1321 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1381 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1441 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1501 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1561 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1621 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1681 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1741 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1801 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1861 aaccgtagtc ggtctcagtc accaggcatc aaataaaacg aaaggctcag tcgaaagact
     1921 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1981 caccttcggg tgggcctttc tgcgttt
//
