LOCUS       sequence-369684-        2574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing cjBlue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_344
FEATURES             Location/Qualifiers
     source          1..2574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(89..193)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      296..884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     785..804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1802..2574,1..19,20..88))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2425..2444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
       61 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      121 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      181 taggggttcc gcgcacattt ccccgaaaag tgccacctgt catgaccaaa atcccttaac
      241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      961 cccctgattc tgtggataac cgtagtcggt ctcctatggc ttccaaaata agcgacaacg
     1021 tacgtatcaa actgtatatg gagggcacgg ttaataatca ccacttcatg tgtgaagcgg
     1081 agggtgaggg caagccatac gaaggaacgc agatggaaaa cattaaagtg accaaaggag
     1141 gcccgctgcc gttctctttt gatatcctga cgccgaactg ccaatatggt tctgtagcca
     1201 taaccaagta cacgtcgggg attccggact attttaaaca gtcattccct gaaggtttta
     1261 cctgggaaag aaccaccatt tatgaagatg gggcttatct gacaactcag caggaaacca
     1321 aacttgatgg aaattgctta gtctacaata ttaaaatcct cggctgcaat tttcccccca
     1381 atggtcctgt tatgcagaaa aaaacgcaag gctgggaacc atgttgcgag atgcgctata
     1441 cacgtgatgg tgtcttgtgc ggtcagacat taatggcact gaaatgtgcc gatgggaacc
     1501 atctgacttg tcatctgcgg actacttacc gatccaaaaa ggcagcgaag gcgttgcaaa
     1561 tgccaccttt ccatttttca gaccatcgtc cggaaattgt gaaggttagc gagaacggca
     1621 cactgtttga gcagcacgaa agtagtgtgg cacgctattg tcagacatgc ccgagcaaac
     1681 ttggtcataa ttaattcttg agaccaagat tatcaaaaag gatcttcacc tagatccttt
     1741 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1801 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1861 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1921 ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa
     1981 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2041 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2101 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2161 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2221 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2281 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2341 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2401 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2461 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2521 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actt
//
