LOCUS       sequence-369685-        2003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing Signal peptide region of CsgA including 
            SecB-N22 sequenced.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_113
FEATURES             Location/Qualifiers
     source          1..2003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(258..1049,1050..1118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     881..900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1119..1223)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1326..1914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1815..1834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggataaccgt agtcggtctc ctatgaaact tttaaaagta gcagcaattg cagcaatcgt
       61 attctccggt agcgctctgg caggtgttgt tcctcagtac ggcggcggcg gtaaccacgg
      121 tggtggcggt aataatagcg gcccaaatgg ttcttgagac caagattatc aaaaaggatc
      181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      361 ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca
      421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      481 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      541 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      601 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      661 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
      721 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
      781 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      841 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
      901 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      961 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1021 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1081 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgt catgaccaaa
     1261 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1321 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1381 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1441 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1501 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1561 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1621 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1681 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1741 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1801 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1861 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1921 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1981 cctgcgttat cccctgattc tgt
//
