LOCUS       sequence-369686-        1902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing 7xhis tag in E1.3b format.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_110
FEATURES             Location/Qualifiers
     source          1..1902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(414..518)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      621..1209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1110..1129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1324..1344
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             complement(join(1455..1902,1..344,345..413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
       61 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      121 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      181 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      241 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      301 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      361 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      421 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      481 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      541 cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
      661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1261 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat
     1321 tctcaccatc accatcacca ccattaagtc aagagaccaa gattatcaaa aaggatcttc
     1381 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1441 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1501 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1561 ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat
     1621 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1681 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1741 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1801 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1861 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg tt
//
