LOCUS       sequence-369687-        2078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing K.rhaeticus native promoter pHxu (200 bases 
            upstream from gene start codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_223
FEATURES             Location/Qualifiers
     source          1..2078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(309..1100,1101..1169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     932..951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1170..1274)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1377..1965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1866..1885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccatcacact tggtgttcct ttctcctgtg gcccccctgt gtctgcggtc ttctagcgcc
       61 cgcaggccgt ttaagctgtc attcatcgac cgtacgcgtt cacttaacgc atagtgcgtc
      121 gctgacgtca taaactaatg acgaaaaaga caatgaccgt cctgctggct gaacgatatt
      181 ctcagctcag gatcagcttc ctggctgaga ccaagattat caaaaaggat cttcacctag
      241 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
      301 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
      361 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
      421 tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca
      481 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      541 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      601 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      661 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      721 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
      781 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
      841 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
      901 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
      961 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     1021 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     1081 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     1141 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     1201 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1261 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa
     1321 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1381 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1441 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1501 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1561 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1621 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1681 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1741 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1801 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1861 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1921 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1981 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2041 tcccctgatt ctgtggataa ccgtagtcgg tctctgac
//
