LOCUS       sequence-369700-        7505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human CD19 antigen in a Sleeping Beauty transposon 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Dobson LJ, Saunderson SC, Smith-Bell SW, McLellan AD
  TITLE     Sleeping Beauty kit sets provide rapid and accessible generation of 
            artificial antigen-presenting cells for natural killer cell 
            expansion.
  JOURNAL   Immunol Cell Biol. 2023 Aug 16. doi: 10.1111/imcb.12679.
  PUBMED    37585342
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Cell Biol. 2023 Aug 16. doi: 10.1111/imcb.12679."
COMMENT     Sequence Label: pSBbi G418 Hu CD19
FEATURES             Location/Qualifiers
     source          1..7505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..60)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    49..273
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        705..809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(810..878,879..1670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1028..1047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2330..2349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2583..2600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2974..2993
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2992..3047
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3093..3112
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3187..3981)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3298..3317)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3908..3927
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        4613..5795
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     5743..5763
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 aggccaagct tggggatcaa ttctctagag ctcgctgatc agcctcgact gtgccttcta
       61 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      121 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
      181 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
      241 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
      301 cctactagat agcggacccc ttaccgaaac atcgccgcat tctgcagagg agtcgaactt
      361 agtgtatgta aacttctgac ccactggaat tgtgatacag tgaattataa gtgaaataat
      421 ctgtctgtaa acaattgttg gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac
      481 tgacttgcca aaactattgt ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt
      541 ttaatgactc caacttaagt gtatgtaaac ttccgacttc aactgtatag ggatccaatt
      601 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
      661 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2581 gcagcgagtc agtgagcgag gaagcggatc cctatacagt tgaagtcgga agtttacata
     2641 caccttagcc aaatacattt aaactcactt tttcacaatt cctgacattt aatcctagta
     2701 aaaattccct gtcttaggtc agttaggatc accactttat tttaagaatg tgaaatatca
     2761 gaataatagt agagagaatg attcatttca gcttttattt ctttcatcac attcccagtg
     2821 ggtcagaagt ttacatacac tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg
     2881 tcccgagtag ctagttcatg gcagccagca tatggcatat gttgccaaac tctaaaccaa
     2941 atactcattc tgatgtttta aatgatttgc cctcccatat gtccttccga gtgagagaca
     3001 caaaaaattc caacacacta ttgcaatgaa aataaatttc ctttattagc cagaagtcag
     3061 atgctcaagg ggcttcatga tgtccccata atttttggca gagggaaaaa gatctcagtg
     3121 gtatttgtga gccagggcat tggccacacc agccaccacc ttctgatagg cagcctgcac
     3181 ctgaggtcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     3241 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     3301 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     3361 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     3421 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     3481 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     3541 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     3601 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     3661 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     3721 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     3781 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     3841 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     3901 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     3961 caatccatct tgttcaatca tggtggcctc aggtgcagag gtttctacag ggaaagagag
     4021 aaaggctaca gttagtttgc tgtgatgatg ctaagtgccg aggtcagatg tctatggatt
     4081 gtacacggaa tcccaacgca ggaaccaaaa aacactcacc caaaaaaatg gcgctaaggc
     4141 cgagagaaag gcgaagttcc gtctacggct atataccttg aaacaggggc ggcagaagtg
     4201 acgcaacgcg gttgcgcgga cggcttcctg tgttttcatt ggtcgaagcg ttctgcgcgt
     4261 gcgcagagat tccccaggga aggcgggccc tcttccgaat gtccaatccg gacattcttc
     4321 cggttggacc tccgcggtca gcgctgtgcc cggcgagaga tcacgtgggg cgcggaggcg
     4381 gtgctgctgg ggcacggccg tccagcctcg gcggccatat ttttgagggg ctgttcatct
     4441 cgttcacacg ctctgtccgc catgtttgtg agtggaagcg ccattacccc ttcaagcgac
     4501 tgaaggctgc agggcctctg gtggcccgca tggggagacc agacccgcca ggcccgcctt
     4561 tccgcactca gtccgggctt actttatttt gtgagacagg gtctcgccta gaggctccgg
     4621 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4681 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4741 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4801 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     4861 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     4921 gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     4981 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg
     5041 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     5101 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     5161 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     5221 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     5281 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     5341 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     5401 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     5461 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     5521 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     5581 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     5641 ttccccatac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     5701 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     5761 ttcaaagttt ttttcttcca tttaaggtgt cgtgaaaact accccaagct ggcctctgag
     5821 gccaccatgc cacctcctcg cctcctcttc ttcctcctct tcctcacccc catggaagtc
     5881 aggcccgagg aacctctagt ggtgaaggtg gaagagggag ataacgctgt gctgcagtgc
     5941 ctcaagggga cctcagatgg ccccactcag cagctgacct ggtctcggga gtccccgctt
     6001 aaacccttct taaaactcag cctggggctg ccaggcctgg gaatccacat gaggcccctg
     6061 gccatctggc ttttcatctt caacgtctct caacagatgg ggggcttcta cctgtgccag
     6121 ccggggcccc cctctgagaa ggcctggcag cctggctgga cagtcaatgt ggagggcagc
     6181 ggggagctgt tccggtggaa tgtttcggac ctaggtggcc tgggctgtgg cctgaagaac
     6241 aggtcctcag agggccccag ctccccttcc gggaagctca tgagccccaa gctgtatgtg
     6301 tgggccaaag accgccctga gatctgggag ggagagcctc cgtgtctccc accgagggac
     6361 agcctgaacc agagcctcag ccaggacctc accatggccc ctggctccac actctggctg
     6421 tcctgtgggg taccccctga ctctgtgtcc aggggccccc tctcctggac ccatgtgcac
     6481 cccaaggggc ctaagtcatt gctgagccta gagctgaagg acgatcgccc ggccagagat
     6541 atgtgggtaa tggagacggg tctgttgttg ccccgggcca cagctcaaga cgctggaaag
     6601 tattattgtc accgtggcaa cctgaccatg tcattccacc tggagatcac tgctcggcca
     6661 gtactatggc actggctgct gaggactggt ggctggaagg tctcagctgt gactttggct
     6721 tatctgatct tctgcctgtg ttcccttgtg ggcattcttc atcttcaaag agccctggtc
     6781 ctgaggagga aaagaaagcg aatgactgac cccaccagga gattcttcaa agtgacgcct
     6841 cccccaggaa gcgggcccca gaaccagtac gggaacgtgc tgtctctccc cacacccacc
     6901 tcaggcctcg gacgcgccca gcgttgggcc gcaggcctgg ggggcactgc cccgtcttat
     6961 ggaaacccga gcagcgacgt ccaggcggat ggagccttgg ggtcccggag cccgccggga
     7021 gtgggcccag aagaagagga aggggagggc tatgaggaac ctgacagtga ggaggactcc
     7081 gagttctatg agaacgactc caaccttggg caggaccagc tctcccagga tggcagcggc
     7141 tacgagaacc ctgaggatga gcccctgggt cctgaggatg aagactcctt ctccaacgct
     7201 gagtcttatg agaacgagga tgaagagctg acccagccgg tcgccaggac aatggacttc
     7261 ctgagccctc atgggtcagc ctgggacccc agccgggaag caacctccct ggggtcccag
     7321 tcctatgagg atatgagagg aatcctgtat gcagcccccc agctccgctc cattcggggc
     7381 cagcctggac ccaatcatga ggaagatgca gactcttatg agaacatgga taatcccgat
     7441 gggccagacc cagcctgggg aggagggggc cgcatgggca cctggagcac caggtgaggc
     7501 ctgtc
//
