LOCUS       sequence-369702-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL1 and ConR2 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT059
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(80..99)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        691..795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(796..864,865..1656)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1014..1033)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(join(2300,1..588))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
       61 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      421 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      661 atgacaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      781 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      841 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      901 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      961 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1021 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1081 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1141 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1201 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1261 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1321 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1381 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1441 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1501 ggtgagcgtg ggtcccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     1561 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1621 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1681 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1741 tttgataatc gcggccgcgg ctcacgtctt atttgggcaa aggagtcagc ggatcaccgt
     1801 actatgtgtg agtgctccta tcgcgcttgc tcctgtgaga tacgatgtgg ccgtgtctga
     1861 ccgtggtgcg tcatctattc actcacttgg ctgcgattta tgctacatgc ccctaatcgg
     1921 ttcctgcagg catactagtc cttcgtctca catcgtagac gggttgggac cgcaggggac
     1981 cccttaacac caactgttgg tgagacggac ttctagaagc tgaattcttc tgtagttcat
     2041 cgggtccgct aactgccttg atctgtcggg tgtctgcttc tctcacaggc ttaggtggat
     2101 ctgctgtccc cagtcagtgc ggtcatcatg cagtcatccg agcgtgtatt gcacacattt
     2161 gtttggcgca tgatgagccg tgatgacccc gtgcggccgc acggttatcc acagaatcag
     2221 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2281 aggccgcgtt gctggcgttt
//
