LOCUS       sequence-369704-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL2 and ConRE connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT060
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(104..692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(184..203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        795..899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(900..968,969..1760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1118..1137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgcacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
       61 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaca ggtggcactt
      781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      901 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      961 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1021 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1081 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1141 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1201 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1261 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1321 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1381 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1441 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1501 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1561 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtccc
     1621 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1681 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1741 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1801 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatcgcggcc
     1861 gctggactgt tgtgatatgc catatatgca gtggtgttcg ccggcgctgg ttccggctgt
     1921 cttgcttagt tgtgagtcgc caggcagtca tcggtatgat ctgtacatga ttcgtcagtg
     1981 tgagcaccac tgacgagcag atttcgctga catgaatatg ctggtccctg cagactgact
     2041 agtgcatcgt ctcatgcttc cagcttcttt gcaccgcctt ggtcgactca ggtccgaccc
     2101 catctgagac gcgattctag agactgaatt cgagctggga gattgtgtct ggcggctcac
     2161 gtcttatttg ggcaaaggag tcagcggatc accgtactat gtgtgagtgc tcctatcgcg
     2221 cttgctcctg tgagatacga tgtggccgtg tctgaccgtg gtgcgtcatc tattcactca
     2281 cttggctgcg atttatgcgg
//
