LOCUS       sequence-369705-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL2 and ConR3 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT061
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(588..1176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(668..687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1279..1383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1384..1452,1453..2244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1602..1621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atttaaaagg atctaggtga agatcctttt tgataatcgc ggccgcgtac tgggttcgag
       61 ttccctgatc cgtatgtcga atggctacca ggctgacgac gatgatatga gcgtgcatcg
      121 gcatcttgcg gtgttgatta gctgccactg gtggctgtcc tacatcagcc tcatgtggtc
      181 taggtctgcg ccattcggtc caggttatga gctgcagcat gactagtact gcgtctcaga
      241 actccagctt ctttgcaccg ccttggtcga ctcaggtccg accccatctg agacggcatt
      301 ctagacgatg aattcgagct gggagattgt gtctggcggc tcacgtctta tttgggcaaa
      361 ggagtcagcg gatcaccgta ctatgtgtga gtgctcctat cgcgcttgct cctgtgagat
      421 acgatgtggc cgtgtctgac cgtggtgcgt catctattca ctcacttggc tgcgatttat
      481 gcggccgcac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1021 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1141 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1201 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gacaggtggc
     1261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1681 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1921 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2101 tcccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2221 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2281 gatttaaaac ttcattttta
//
