LOCUS       sequence-369706-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL3 and ConRE connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT062
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(361..949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(441..460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1052..1156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1157..1225,1226..2017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1375..1394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catgcgtctc atgctgccat tcttcgataa ccgctcgtaa gttctgcggt cggcgatgaa
       61 ctgagacgac tgtctagagc atgaattcgt ggctttacct cgtattgcgc gtactgggtt
      121 cgagttccct gatccgtatg tcgaatggct accaggctga cgacgatgat atgagcgtgc
      181 atcggcatct tgcggtgttg attagctgcc actggtggct gtcctacatc agcctcatgt
      241 ggtctaggtc tgcgcggccg cacggttatc cacagaatca ggggataacg caggaaagaa
      301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1021 catgacaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1081 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1141 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1201 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1261 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1321 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1381 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1441 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1501 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1561 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1621 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1681 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1741 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1801 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1861 cggtgagcgt gggtcccgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1921 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1981 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2041 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2101 ttttgataat cgcggccgct ggactgttgt gatatgccat atatgcagtg gtgttcgccg
     2161 gcgctggttc cggctgtctt gcttagttgt gagtcgccag gcagtcatcg gtatgatctg
     2221 tacatgattc gtcagtgtga gcaccactga cgagcagatt tcgctgacat gaatatgctg
     2281 gtccctgcag aaggactagt
//
