LOCUS       sequence-369707-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL3 and ConR4 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT063
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(514..1305,1306..1374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1137..1156
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1375..1479)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1582..2170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2071..2090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatgtaggac agccaccagt ggcagctaat caacaccgca agatgccgat gcacgctcat
       61 atcatcgtcg tcagcctggt agccattcga catacggatc agggaactcg aacccagtac
      121 gcgcaatacg aggtaaagcc acgaattcca gttctagaca tgcgtctcag ttcatcgccg
      181 accgcagaac ttacgagcgg ttatcgaaga atggcggtat gagacgcgat actagtcgta
      241 ctgcaggaat ctttactccc aagcgagcgc ctgcactaga cgaactaggc aagatgcgtc
      301 caatccgtct aaacatggtg acaacgctgg acagatgacg taacaccgag ccacatcctg
      361 aaatcgaggc aggctaaccg aaaccgtgac aatgcaaaga gacagcctga cgcggccgcg
      421 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      481 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      541 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      601 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc
      661 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      721 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      781 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      841 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      901 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      961 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1021 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1081 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1141 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1201 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1261 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1321 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1381 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1441 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1501 acctgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcgca
     2281 gacctagacc acatgaggct
//
