LOCUS       sequence-369709-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL4 and ConR5 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT065
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     357..376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1100..1891,1892..1960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1723..1742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1961..2065)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(2168..2300,1..456)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
       61 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      541 tctgtggata accgtgcggc cgcgcctgca ctagacgaac taggcaagat gcgtccaatc
      601 cgtctaaaca tggtgacaac gctggacaga tgacgtaaca ccgagccaca tcctgaaatc
      661 gaggcaggct aaccgaaacc gtgacaatgc aaagagacag cctgacgctg cttggtgtct
      721 cgtattccga attccgattc tagacgtacg tctcaggtag agttccccgg ttacctctcc
      781 acgcggtcta accatttggg aaagttgaga cgctagacta gtctgactgc agatggacga
      841 aatgtttcac gagcacgcac aaggtcaggg cactcatgcg acaatcaact cgatgcatga
      901 tccgcaccat tgtcgagggg ccagcgtcaa tagtgccgat gaccacagac ccggttaaga
      961 catagccgaa tggagccgcg ccgaccacag aatgatagcg gccgcgatta tcaaaaagga
     1021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1201 agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc
     1261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gtcatgacca
     2101 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2161 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2221 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2281 ctggcttcag cagagcgcag
//
