LOCUS       sequence-369710-        6630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble expression of human 4-1BB (CD137)-Fc. Human IgG1 Fc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6630)
  TITLE     McLellan Lab Plasmids 2022
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1(-) Hu 4-1BB(CD137)-Fc
FEATURES             Location/Qualifiers
     source          1..6630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1202..1219)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1208..1432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1478..1906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1565..1584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1775..1796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1915..1935)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1920..2249
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2100..2235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2162..2181
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2316..3110
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2370..2389)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2980..2999
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3284..3405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3321..3340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3375..3394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3454..3470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3454..3470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3467..3489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3478..3494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3502..3508,3509..3526,3527..3532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3547..3568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3685..3702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3856..4444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3936..3955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4615..5406,5407..5475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5238..5257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5476..5580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5655..5674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5846..6225
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6226..6429
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6380..6400
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6474..6493
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6474..6492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagtttgcag gatccttgta gtaactgccc agctggtaca ttctgtgata ataacaggaa
       61 tcagatttgc agtccctgtc ctccaaatag tttctccagc gcaggtggac aaaggacctg
      121 tgacatatgc aggcagtgta aaggtgtttt caggaccagg aaggagtgtt cctccaccag
      181 caatgcagag tgtgactgca ctccagggtt tcactgcctg ggggcaggat gcagcatgtg
      241 tgaacaggat tgtaaacaag gtcaagaact gacaaaaaaa ggttgtaaag actgttgctt
      301 tgggacattt aacgatcaga aacgtggcat ctgtcgaccc tggacaaact gttctttgga
      361 tggaaagtct gtgcttgtga atgggacgaa ggagagggac gtggtctgtg gaccatctcc
      421 agccgacctc tctccgggag catcctctgt gaccccgcct gcccctgcga gagagccagg
      481 acactctccg caggacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg
      541 gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac
      601 ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa
      661 ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta
      721 caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg
      781 caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat
      841 ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga
      901 ggagatgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga
      961 catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc
     1021 cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag
     1081 gtggcagcag gggaacgtct tctcatgctc cgtgatgcac gaggctctgc acaaccacta
     1141 cacgcagaag agcctctccc tgtctccggg taaataagct taagtttaaa ccgctgatca
     1201 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1261 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1321 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1381 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     1441 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     1501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     1921 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1981 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2041 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2161 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     2221 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     2281 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     2341 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     2401 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     2461 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     2521 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     2581 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     2641 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2701 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2761 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     2821 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     2881 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     2941 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3001 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3061 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     3121 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     3181 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     3241 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     3301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     3421 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3481 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3541 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3601 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3661 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3721 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3781 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3841 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3901 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3961 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4021 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4081 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4141 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4201 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4261 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4321 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4381 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4441 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4501 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4561 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4621 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4681 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4741 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4801 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4861 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4921 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4981 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5041 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5101 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5161 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5221 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5281 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5341 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5401 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5461 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5521 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5581 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     5641 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     5701 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     5761 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     5821 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     5881 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5941 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6001 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6061 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6121 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6181 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6241 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6301 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6361 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6421 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     6481 actcactata gggagaccca agctggctag cgtttaaacg ggccctctag actcgagcgg
     6541 ccgccactgt gctggatatc tgcagaattc caccatgcga aggatgcaac ttcttctgct
     6601 catcgcactt tccttggcgc tggtgacaaa
//
