LOCUS       sequence-369711-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL5 and ConRE connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT066
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     346..365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1089..1880,1881..1949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1712..1731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1950..2054)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(2157..2300,1..445)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
       61 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      241 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      481 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      541 ccgtgcggcc gcacgcacaa ggtcagggca ctcatgcgac aatcaactcg atgcatgatc
      601 cgcaccattg tcgaggggcc agcgtcaata gtgccgatga ccacagaccc ggttaagaca
      661 tagccgaatg gagccgcgcc gaccacagaa tgatagcggt gtagcgaagg atcaagggaa
      721 ttcctagtct agactgacgt ctcaaagtta ccatggtcgt aaaagcctcc cacggtcacg
      781 ggacactcgc agcatgagac ggactactag taaccctgca gggaccagca tattcatgtc
      841 agcgaaatct gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg
      901 actgcctggc gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg
      961 catatatggc atatcacaac agtccagcgg ccgcgattat caaaaaggat cttcacctag
     1021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1201 tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca
     1261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     1381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     1441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     1501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     1561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     1621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     1681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     1741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     1801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     1861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     1921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     1981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa
     2101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2281 agagcgcaga taccaaatac
//
