LOCUS       sequence-369714-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL6 and ConR7 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT069
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(770..1358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(850..869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1461..1565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1566..1634,1635..2300,1..126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1784..1803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
       61 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      121 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
      181 taatttaaaa ggatctaggt gaagatcctt tttgataatc gcggccgcgc tcgtcaactg
      241 tagacccgaa aatcgtatct gtgaggaacg ccgtgcgaac cctttaccga gaggtggagc
      301 gagtagtatc ccgtccgtag aatctggtgc ttccctgaac tgcgtgaaat agtcaacagc
      361 cgacaataac tgccacaatg aatagtccga ccgctgcagt tccactagta caccgtctca
      421 tcgtgatgct gggcataaac cgccgattgg agtagtgagc atctactcta tgagacgtgt
      481 gtctagaagg agaattcaca tggtgactcg tccttctgcg tcgtgtgctc cgagatagtg
      541 gttccgttac cgtagaccga taccttccca acttcgcttt acaatagcct cgtgaataat
      601 cagggtgact cagacacagt aggagactta cgccagagca gataactttc gtttaccgca
      661 gggcggccgc acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgacaggtg
     1441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1561 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1621 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1681 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1741 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1801 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1861 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1921 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1981 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2041 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2101 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2161 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2221 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2281 ggtcccgcgg tatcattgca
//
