LOCUS       sequence-369716-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL7 and ConRE connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT070
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(900..1488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(980..999)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1591..1695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1696..1764,1765..2300,1..256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1914..1933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
       61 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg
      121 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
      181 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
      241 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
      301 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc gcggccgctg
      361 gactgttgtg atatgccata tatgcagtgg tgttcgccgg cgctggttcc ggctgtcttg
      421 cttagttgtg agtcgccagg cagtcatcgg tatgatctgt acatgattcg tcagtgtgag
      481 caccactgac gagcagattt cgctgacatg aatatgctgg tccctgcagt cctactagtg
      541 aagcgtctca tgctgcatgg cgggcataac cgcgataatt ctacgtatcc gtccactcgt
      601 tgagacgaca ctctagatgt ggaattcagc acctttccca cgtaaacgcg ctcgtcaact
      661 gtagacccga aaatcgtatc tgtgaggaac gccgtgcgaa ccctttaccg agaggtggag
      721 cgagtagtat cccgtccgta gaatctggtg cttccctgaa ctgcgtgaaa tagtcaacag
      781 ccgacaataa ctgcggccgc acggttatcc acagaatcag gggataacgc aggaaagaac
      841 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      901 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      961 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1021 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1081 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1141 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1201 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1321 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1381 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1441 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1501 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1561 atgacaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1621 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1681 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1741 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1801 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1861 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1921 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     1981 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2041 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2101 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2161 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2221 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2281 ctacttactc tagcttcccg
//
