LOCUS       sequence-369719-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL7 and ConR8 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT071
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(573..1364,1365..1433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1196..1215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1434..1538)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1641..2229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2130..2149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttatcccct gattctgtgg ataaccgtgc ggccgcagtt attgtcggct gttgactatt
       61 tcacgcagtt cagggaagca ccagattcta cggacgggat actactcgct ccacctctcg
      121 gtaaagggtt cgcacggcgt tcctcacaga tacgattttc gggtctacag ttgacgagcg
      181 cgtttacgtg ggaaaggtgc tgaattcgtg ttctagactt ccgtctcaac gagtggacgg
      241 atacgtagaa ttatcgcggt tatgcccgcc atgcctcctg agacgaggaa ctagtacacc
      301 tgcagcagtc catccgatcg tacatgccgt attctatttc gtcgggagag ttcagtcaca
      361 ggaccgtgtc tacagataag gcaatcgtgc gttgggcagt ttttactatt ccagggactt
      421 cttcggcgag aggagcaggt caatccgttt cgtattactg aggttggtag gcggccgcga
      481 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      541 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      601 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      661 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca
      721 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      781 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      841 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      901 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      961 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1021 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1081 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1141 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1201 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1261 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1321 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1381 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1441 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1501 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1561 cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1621 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1681 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1741 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1801 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1861 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1921 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1981 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2041 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2101 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2161 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2221 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2281 gctcacatgt tctttcctgc
//
