LOCUS       sequence-369726-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette with ConL8 and ConR9 connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT073
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(227..815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(307..326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        918..1022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1023..1091,1092..1883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1241..1260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggattgacct gctcctctcg ccgaagaagt ccctggaata gtaaaaactg cccaacgcac
       61 gattgcctta tctgtagaca cggtcctgtg actgaactct cccgacgaaa tagaatacgg
      121 cggccgcacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      721 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      781 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      841 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg acaggtggca
      901 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      961 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1021 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1081 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1141 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1201 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1261 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1321 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1381 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1441 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1501 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1561 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1621 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1681 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1741 cccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1801 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1861 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1921 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatcgcg
     1981 gccgcgcgga atcaccgagc agtcctctac tcctacaatc cacgagaacg gcgaatacag
     2041 ttacgaacag aagtttaccc gaaaagtcag cctcccttac tatcccagat tatcagtcta
     2101 ttgctccaac ctgcggacag cgtgtgctgc cgatttggca cagttagtca ctgcagcaca
     2161 actagtcaac cgtctcaccc tgactggttt cgtctaccgc catgctacca ccaaatacgg
     2221 attggagtga gacggtgttc tagagttgga attcgtccgt cattgcatct aggtgcctac
     2281 caacctcagt aatacgaaac
//
