LOCUS       sequence-36973-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA
  TITLE     How capping protein binds the barbed end of the actin filament.
  JOURNAL   Curr Biol. 2003 Sep 2. 13(17):1531-7.
  PUBMED    12956956
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Sep 2. 13(17):1531-7."
COMMENT     Sequence Label: pet 3d alpha 1 beta 2 CP
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             323..345
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(376..395)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(377..395)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     483..501
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gggcntgctt cttcgtaatg atcaacttaa aggaaatttg gtttcttgga tctaaaaatc
       61 tgctgttacc aaggtcacca tgctctgtaa ttaagacctg gtcatcgtag ccttctatct
      121 tcacgggtgt gaactgatcc atgttatact gggcaaatgc atgtgctgcc ccttccctga
      181 ggagattgtc attgttaagc agtagccgga catcattgaa tacttcatta aactcccctg
      241 ggggtgcatg ggtgatgaat ttagcggcta tgcggacctt ctcttcatcc gacacccgat
      301 cctcaaagtc agccatggta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      361 aaaccgttgt ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatcatccgg
      421 atatagttcc tcctttcagc aaaaaacccc tcaaganccg tttagangnc ccaaggggtt
      481 atgctagtta ttgctcagcg gntcaacact gctgctttct cttcanggct ccaccaggtc
      541 gtnctnaacg cntcntgctt tgattttctg naaangtctg nccagacctc ancccgtnga
      601 aangtccntt gnttttccaa gnaaattcat ncacgngctn cggantttnt ttccagnncc
      661 ccncnaggnc ccnagttngc nattgtgggg naaaatcccc
//
