LOCUS       sequence-369737-        8750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human 41BBL, CD86, and membrane bound human IL-15 
            (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in 
            artificial antigen presenting cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Dobson LJ, Saunderson SC, Smith-Bell SW, McLellan AD
  TITLE     Sleeping Beauty kit sets provide rapid and accessible generation of 
            artificial antigen-presenting cells for natural killer cell 
            expansion.
  JOURNAL   Immunol Cell Biol. 2023 Aug 16. doi: 10.1111/imcb.12679.
  PUBMED    37585342
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Cell Biol. 2023 Aug 16. doi: 10.1111/imcb.12679."
COMMENT     Sequence Label: pSBbi 41BBL-CD86 mIL-15
FEATURES             Location/Qualifiers
     source          1..8750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             779..835
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1832..1888
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(2588..2607)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2653..2708
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2707..2726)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3100..3117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3271..3859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3351..3370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4030..4821,4822..4890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4653..4672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4891..4995)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5427..5651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5640..5657
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        complement(6940..8122)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     complement(6972..6992)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggccaccatg gaatacgcct ctgacgcttc actggacccc gaagccccgt ggcctcccgc
       61 gccccgcgct cgcgcctgcc gcgtactgcc ttgggccctg gtcgcggggc tgctgctgct
      121 gctgctgctc gctgccgcct gcgccgtctt cctcgcctgc ccctgggccg tgtccggggc
      181 tcgcgcctcg cccggctccg cggccagccc gagactccgc gagggtcccg agctttcgcc
      241 cgacgatccc gccggcctct tggacctgcg gcagggcatg tttgcgcagc tggtggccca
      301 aaatgttctg ctgatcgatg ggcccctgag ctggtacagt gacccaggcc tggcaggcgt
      361 gtccctgacg gggggcctga gctacaaaga ggacacgaag gagctggtgg tggccaaggc
      421 tggagtctac tatgtcttct ttcaactaga gctgcggcgc gtggtggccg gcgagggctc
      481 aggctccgtt tcacttgcgc tgcacctgca gccactgcgc tctgctgctg gggccgccgc
      541 cctggctttg accgtggacc tgccacccgc ctcctccgag gctcggaact cggccttcgg
      601 tttccagggc cgcttgctgc acctgagtgc cggccagcgc ctgggcgtcc atcttcacac
      661 tgaggccagg gcacgccatg cctggcagct tacccagggc gccacagtct tgggactctt
      721 ccgggtgacc cccgaaatcc cagccggact cccttcaccg aggtcggaag gatccggagc
      781 tactaacttc agcctgctga agcaggctgg tgacgtcgag gagaatcctg gccccatgga
      841 tccccagtgc actatgggac tgagtaacat tctctttgtg atggccttcc tgctctctgg
      901 tgctgctcct ctgaagattc aagcttattt caatgagact gcagacctgc catgccaatt
      961 tgcaaactct caaaaccaaa gcctgagtga gctagtagta ttttggcagg accaggaaaa
     1021 cttggttctg aatgaggtat acttaggcaa agagaaattt gacagtgttc attccaagta
     1081 tatgggccgc acaagttttg attcggacag ttggaccctg agacttcaca atcttcagat
     1141 caaggacaag ggcttgtatc aatgtatcat ccatcacaaa aagcccacag gaatgattcg
     1201 catccaccag atgaattctg aactgtcagt gcttgctaac ttcagtcaac ctgaaatagt
     1261 accaatttct aatataacag aaaatgtgta cataaatttg acctgctcat ctatacacgg
     1321 ttacccagaa cctaagaaga tgagtgtttt gctaagaacc aagaattcaa ctatcgagta
     1381 tgatggtatt atgcagaaat ctcaagataa tgtcacagaa ctgtacgacg tttccatcag
     1441 cttgtctgtt tcattccctg atgttacgag caatatgacc atcttctgta ttctggaaac
     1501 tgacaagacg cggcttttat cttcaccttt ctctatagag cttgaggacc ctcagcctcc
     1561 cccagaccac attccttgga ttacagctgt acttccaaca gttattatat gtgtgatggt
     1621 tttctgtcta attctatgga aatggaagaa gaagaagcgg cctcgcaact cttataaatg
     1681 tggaaccaac acaatggaga gggaagagag tgaacagacc aagaaaagag aaaaaatcca
     1741 tatacctgaa agatctgatg aagcccagcg tgtttttaaa agttcgaaga catcttcatg
     1801 cgacaaaagt gatacatgtt ttggatccgg agctactaac ttcagcctgc tgaagcaggc
     1861 tggtgacgtc gaggagaatc ctggccccat gacagaatac aagcctacag ttagactggc
     1921 aacacgcgat gatgttccca gggcggttcg gacacttgct gctgctttcg ccgattatcc
     1981 ggccacgcga cacactgtgg acccagatag gcacatcgag agagtgacgg agctgcaaga
     2041 gctgtttttg actagggttg gacttgatat cggaaaagtt tgggttgctg atgatggtgc
     2101 tgcggtagcg gtctggacaa ctcctgagtc agtggaggcc ggagcggtct tcgctgagat
     2161 cggtcctcgc atggccgaat tgagcggaag tcgattggcc gctcagcagc aaatggaggg
     2221 tcttctggca ccccacaggc ctaaggagcc tgcgtggttt cttgcaacag tgggcgtgtc
     2281 tccagatcat caggggaaag gtcttggttc tgcagtagtg ctgcccggtg tagaggcagc
     2341 ggagagggca ggcgtaccag cgttcttgga aacctcagcc ccgagaaacc tgcctttcta
     2401 tgagcgcctc gggtttaccg tcacagcgga tgttgaagtc cctgagggac cgcgcacatg
     2461 gtgtatgacc cgaaagcccg gtgcctgatt cgaaggcctg tcgtgaattc actcctcagg
     2521 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     2581 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     2641 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     2701 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     2761 tggtttagag tttggcaaca tatgccatat gctggctgcc atgaactagc tactcgggac
     2821 cccttaccga aacatcgccg cattctgcag aggagtcgag tgtatgtaaa cttctgaccc
     2881 actgggaatg tgatgaaaga aataaaagct gaaatgaatc attctctcta ctattattct
     2941 gatatttcac attcttaaaa taaagtggtg atcctaactg acctaagaca gggaattttt
     3001 actaggatta aatgtcagga attgtgaaaa agtgagttta aatgtatttg gctaaggtgt
     3061 atgtaaactt ccgacttcaa ctgtataggg atccgcttcc tcgctcactg actcgctgcg
     3121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3901 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3961 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4021 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4081 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4141 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4201 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4261 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4321 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4381 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4441 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4501 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4561 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4621 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4681 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4741 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4801 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4861 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4921 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4981 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc
     5041 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
     5101 attggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
     5161 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
     5221 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
     5281 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
     5341 agttcgactc ctctgcagaa tgcggcgatg tttcggtaag gggtccgcta tctagtaggc
     5401 ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct
     5461 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     5521 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     5581 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     5641 agaaggcaca gtcgaggctg atcagcgagc tctagagaat tgatccccaa gcttggcctg
     5701 acaggcctta gagatggtgt gaacaatttt ccagatcctc gtcccggcta ctagtccccc
     5761 acgtgactgg caaggcttcc atggcttcca tctcaacgct ggcaaggggt ggagtctgcc
     5821 tggatttcaa gtagcaagcc aacagggata cagcagagag cccacacaac aggacggtgc
     5881 ttgtgctaat agctactgtt gtatcggaat ggccctgggg atataccccc ggtggctggt
     5941 gtgaagcgga ggccgtcagt tcccaattct tcgccgtggt ttgggaaggg gtcccatggc
     6001 ttgactcgtg tgaactgatt tctgtggtcc cagtgctagg ggatttagaa ggcataagtt
     6061 gagagccggg aacaatggct gccgtggtgg cggcagtatt gttagaagac ggagatgacg
     6121 cggcaggttc tttaccggac ggagacaaag actcaggctg tggtgtgaca ccagcggtag
     6181 tcaccgttga cggaggagca ggcctttggt gtaccaacgc gggatctcga atacatttca
     6241 atgatggagt cgtccaatgg gccacatttg tcgctttgtt gagtacacac tctgtaagtg
     6301 aactagtgcc agccttccgt ttgaaaccgc tattacagat gtatctttcc cgactgtaga
     6361 ggctgtaact tttaacccag atatcagcgt gttcgacaga catcggcgga gggcacgtga
     6421 tctgaaggga cccgcctccg ctgccgccgc cgccgctccc tcccccacca gatcccccgc
     6481 ccccacttcc gccccctgag ctggtattga tgaacatctg aactatatgc acgaagctct
     6541 gcaaaaattc tttaatattc ttttcctcga gttcctcaca ttctttacag ccagactcgg
     6601 taacattacc attactactc aaagagttat tggcgagtat aatcagattt tcgacagtat
     6661 catgtatact tgcatcacca gattccaggc ttatgacttg aagttccaag aggaagcact
     6721 tcatcgctgt cactttacag gatggatgca catctgattc cgtgtagagg gtagcgtcta
     6781 tgtgcataga ttggatcagg tcctcgattt ttttcaagtc ggaaatcacg ttcacccaat
     6841 tactcattat aactgacgcg gagataagca agaagctaaa gatttgtacc tgaaagtcca
     6901 tggtggaatt cggcctcaga ggccagcttg gggtagtttt cacgacacct taaatggaag
     6961 aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag
     7021 ggcaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca
     7081 ctcagtatgg ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact
     7141 ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca
     7201 tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg
     7261 ctctcccgag cgccgcgtcc tccattttga gctccctgca gcagggccgg gaagcggcca
     7321 tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat
     7381 acacggcggc gcgaggccag gcaccagagc aggccggcca gcttgagact acccccgtcc
     7441 gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct gggacgcacg
     7501 ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc
     7561 atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat
     7621 ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg
     7681 gcggccccag cgcccaagcc aggcctcaac tcaagcacga ggcgaagggg ctccttaagc
     7741 gcaaggcctc gaactctccc acccacttcc aacccgaagc tcgggatcaa gaatcacgta
     7801 ctgcagccag gggcgtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc
     7861 caggcccgcg ggaaccacac acggcactta cctgtgttct ggcggcaaac ccgttgcgaa
     7921 aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa
     7981 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
     8041 cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
     8101 cccactgacg ggcaccggag cctctaggcg agaccctgtc tcacaaaata aagtaagccc
     8161 ggactgagtg cggaaaggcg ggcctggcgg gtctggtctc cccatgcggg ccaccagagg
     8221 ccctgcagcc ttcagtcgct tgaaggggta atggcgcttc cactcacaaa catggcggac
     8281 agagcgtgtg aacgagatga acagcccctc aaaaatatgg ccgccgaggc tggacggccg
     8341 tgccccagca gcaccgcctc cgcgccccac gtgatctctc gccgggcaca gcgctgaccg
     8401 cggaggtcca accggaagaa tgtccggatt ggacattcgg aagagggccc gccttccctg
     8461 gggaatctct gcgcacgcgc agaacgcttc gaccaatgaa aacacaggaa gccgtccgcg
     8521 caaccgcgtt gcgtcacttc tgccgcccct gtttcaaggt atatagccgt agacggaact
     8581 tcgcctttct ctcggcctta gcgccatttt tttgggtgag tgttttttgg ttcctgcgtt
     8641 gggattccgt gtacaatcca tagacatctg acctcggcac ttagcatcat cacagcaaac
     8701 taactgtagc ctttctctct ttccctgtag aaacctctgc acctgagggc
//
