LOCUS       sequence-369756-        5270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from Escherichia coli M885 prophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_prophage_Escherichia_coli_2
FEATURES             Location/Qualifiers
     source          1..5270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    37..177
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(103..122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     263..285
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(441..1319)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1346..1630
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1553..1572
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     regulatory      1848..1857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             2825..2836
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3364..3391
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3410..3501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3502..3570,3571..4362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3720..3739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4533..5121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5022..5041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
       61 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      121 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      181 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      241 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      301 ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca tgcataatgt
      361 gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca
      421 attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca
      481 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa
      541 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg
      601 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc
      661 cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg
      721 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg
      781 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt
      841 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg
      901 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga
      961 aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc
     1021 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga
     1081 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc
     1141 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc
     1201 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc
     1261 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata
     1321 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
     1381 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa
     1441 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
     1501 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
     1561 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
     1621 gtttctccat acccgttttt tgggctaaca ggaggaatta accatggcgg agaccaccaa
     1681 ggaacgtctg aacaaacaat tcgcgcagct ggagagcgaa cgtcagagct ttgagccgca
     1741 ctggcgtgaa ctgagcgatt acattaaccc gcgtggtagc cgtttcctga ccagcgaggt
     1801 gaaccgtaac gatcgtcgta acacccgtat cattgacagc accggtacca tggcggcgcg
     1861 taccctggcg agcggtatga tgagcggtat caccagcccg gcgcgtccgt ggttccgtct
     1921 ggcgaccccg gatccggaga tgatggacta cggtccggtg aagctgtggc tggaagcggt
     1981 tcagaaccgt atgaacgaca tgtttaacaa aagcaacctg tatcaaagcc tgccgcagct
     2041 gtacggtagc ctgggcacct atagcaccgg tgcgatggcg gtgctggagg atgatgaaga
     2101 tatcattcgt accatgccgt tcccgattgg tagctactat ctggcgaaca gcccgcgtgg
     2161 cagcgttgac acctgcttcc gtaagtttag catgaccgtg cgtcaactgg ttcaggagtt
     2221 tggtctgaac aacgtgagcg agagcgttaa gagcatgtgg gaaagcggca cctacgagaa
     2281 atggattgaa gtgatgcaca gcgtttatcc gaacatcgac cgtgatacca gcaagctgga
     2341 tagcaagaac aaaccgttta aaagcgtgta ctatgaagtt ggtggcgaca acgataagct
     2401 gctgcgtgag agcggtttcg atgaatttcc gattatggcg ccgcgttggg aagtgaacgg
     2461 tgaagacgtt tacggcagca gctgcccggg tatgctggcg ctgggtccgg tgaaagcgct
     2521 gcaactgctg cagaagcgta aaagccaact gatcgataaa gcgaccaacc cgccgatggt
     2581 tgcgccgacc agcctgaaaa accagcgtgc gagcctgctg ccgggtgata tcacctacat
     2641 tgaccaaatc accggtcagg atggcttccg tccggcgtat ctggtgaacc cgagcaccgc
     2701 ggacctggtt gcggacatcc aagatacccg tcagatcatt aacagcgcgt acttcgttga
     2761 cctgtttatg atgctgcaga acattaacac ccgtagcatg ccggtggagg cggttatcga
     2821 aatgaaagag gagaaactgc tgatgctggg tccggtgctg gagcgtctga acgatgaatg
     2881 cctgaacccg ctgattgacc gtgcgtttag catgatggtt cgtaaaaaca tgctgccgcc
     2941 gccgccggat gcgatggagg gcatgccgct gaaggtggaa tatattagcg ttatggcgca
     3001 agcgcagaaa agcatcggtc tgagcagcct ggcgagcacc gtgaacttca ttggtcaact
     3061 ggcgcaggcg aagccggagg cgctggacaa actgaacgtt gaccaagcga ttgatgcgtt
     3121 tgcggacatg agcggtgtga gcccgaccgt gatcgttccg caagagcagg ttgaacaagc
     3181 gcgtcagcaa cgtgcgcagc aacagcaaca gcaacagatg atggcgatgg gtatggcggc
     3241 ggcgcagggt gcgaagaccc tgagcgaagc gaaaaccagc gatccgagcg tgctgagcgc
     3301 gatggcgaac gcggttagcg gtcagggtgg ccaaagccag taatgccagg catcaaatta
     3361 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
     3421 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3481 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3601 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3661 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3721 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     3781 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3841 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3901 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3961 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4021 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4081 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4141 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4201 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4261 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4321 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4381 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4441 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4501 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4561 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4621 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     4681 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4741 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4801 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4861 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4921 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4981 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5041 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5101 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5161 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5221 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
//
