LOCUS       sequence-369764-        3681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC30A7_STOP
FEATURES             Location/Qualifiers
     source          1..3681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1238..1337)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1354..1373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1355..1373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1378..1394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1378..1394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1496..1515)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1507..2316
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1574..1593)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2463..3051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2952..2971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3205..3222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3381..3408)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3500..3586)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3635..3657
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3649..3666
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3650..3666
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgct gcccctgagc
      121 atcaaggacg atgagtacaa gccccctaag tttaacctgt tcggcaagat ctctggctgg
      181 ttccggtcca tcctgtctga caagaccagc agaaacctgt tctttttcct gtgcctgaat
      241 ctgtcctttg ccttcgtgga gctgctgtat ggcatctggt ctaattgtct gggcctgatc
      301 agcgactcct ttcacatgtt tttcgattct acagcaatcc tggcaggact ggcagcaagc
      361 gtgatctcca agtggcggga caacgatgcc tttagctacg gctatgtgag agccgaggtg
      421 ctggccggct tcgtgaatgg cctgtttctg atcttcaccg cctttttcat cttcagcgag
      481 ggagtggaga gggccctggc accacccgat gtgcaccacg agcgcctgct gctggtgtcc
      541 atcctgggct ttgtggtgaa cctgatcggc atcttcgtgt tcaagcacgg aggacacgga
      601 cactctcacg gaagcggcca tggacactct cacagcctgt tcaatggcgc cctggaccag
      661 gcacacggac acgtggatca ctgccactcc cacgaggtga agcacggagc agcacactct
      721 cacgaccacg cccacggcca cggccacttt cactcccacg atggcccctc tctgaaggag
      781 accacaggcc ctagcaggca gatcctgcag ggcgtgttcc tgcacatcct ggccgacaca
      841 ctgggaagca tcggcgtgat cgcatccgcc atcatgatgc agaactttgg cctgatgatc
      901 gccgatccaa tctgttccat cctgatcgcc atcctgatcg tggtgtctgt gatccccctg
      961 ctgagggaga gcgtgggcat cctgatgcag cgcacccctc cactgctgga gaattctctg
     1021 cctcagtgct accagagggt gcagcagctg cagggcgtgt atagcctgca ggagcagcac
     1081 ttctggaccc tgtgctccga cgtgtacgtg ggcacactga agctgatcgt ggcaccagac
     1141 gcagatgcaa ggtggatcct gtcccagacc cacaacatct ttacacaggc cggcgtgaga
     1201 cagctgtatg tgcagatcga tttcgccgcc atgtaagacc cagctttctt gtacaaagtt
     1261 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1321 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1381 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1561 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1621 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1681 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1741 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1801 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1861 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1921 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1981 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2041 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2101 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2161 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2221 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2281 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2341 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2401 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2461 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2521 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2581 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2641 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2701 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2761 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2821 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2881 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2941 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3001 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3061 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3121 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3181 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3241 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3301 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3361 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3421 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3481 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3541 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3601 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3661 gccagtctta agctcgggcc c
//
