LOCUS       sequence-369766-       13574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  two component reporter construct for Arabidopsis UBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13574)
  TITLE     Britt Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AtUBQ10+pLB12
FEATURES             Location/Qualifiers
     source          1..13574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    253..277
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    303..327
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1367..1391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1420..1466
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             1495..1746
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             1762..1995
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             2005..2940
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2053..2946
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        3912..4091
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4141..4944
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4204..4223)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4814..4833
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4976..5228
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5302..5326
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     rep_origin      5413..6048
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(6723..7511)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8608..9196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9097..9116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9350..9367
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(10556..11704)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  12329..13096
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            13156..13265
                     /note="incP origin of transfer"
ORIGIN
        1 acggccgcga cctccgcgat cgcggcaaag tatctggccc gtaaagattc gcgcacgatg
       61 gcgctgatcg gcaacggtgc ccaaagtgag ttccaggctt tggccttcaa ggcgctgatc
      121 ggcgtcgatc ggatacgact ttacgacatc gaccccgagg ctactgctcg ctgctcccgc
      181 aatctccagc ggttcgggtt tcagatcgag gcctgcacct ctgcggaaca ggcggtcgaa
      241 ggtgccgata tcgtttaccc gccaatatat cctgtcaaag tcgaggcgcg ccaagctatc
      301 aaacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccgat caggatattc
      361 ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag gaccggataa
      421 gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc ttgttacatt
      481 gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa atatggacca
      541 ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc gagtcaccaa
      601 accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc attatcctat
      661 gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag aaatggataa
      721 tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact gattttataa
      781 gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat aaagcacgaa
      841 gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc aattattgcc
      901 aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa aatataaatc
      961 gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa ttgtggttgt
     1021 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
     1081 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
     1141 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
     1201 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
     1261 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
     1321 tttctctcaa ttctctctac cgtgatcaag aagggtgggc gcgccgaccc agctttcttg
     1381 tacaaagtgg ttcgataatt cttaattaac tagtccttcg caagaccctt cctctatata
     1441 aggaagttca tttcatttgg agaggacacg ctgggatccc caattccggg cggaatgaaa
     1501 gcgttaacgg ccaggcaaca agaggtgttt gatctcatcc gtgatcacat cagccagaca
     1561 ggtatgccgc cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc cccaaacgcg
     1621 gctgaagaac atctgaaggc gctggcacgc aaaggcgtta ttgaaattgt ttccggcgca
     1681 tcacgcggga ttcgtctgtt gcaggaagag gaagaagggt tgccgctggt aggtcgtgtg
     1741 gctgccggtg aaccgtcgag cgcccccccg accgatgtca gcctggggga cgagctccac
     1801 ttagacggcg aggacgtggc gatggcgcat gccgacgcgc tagacgattt cgatctggac
     1861 atgttggggg acggggattc cccgggtccg ggatttaccc cccacgactc cgccccctac
     1921 ggcgctctgg atatggccga cttcgagttt gagcagatgt ttaccgatgc ccttggaatt
     1981 gacgagtacg gtggggatcc gtctgctgga gacatgagag ctgccaacct ttggccaagc
     2041 ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag
     2101 atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc
     2161 agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg
     2221 gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat
     2281 caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc
     2341 tccatggagc acccagggaa gctactgttt gctcctaact tgctcttgga caggaaccag
     2401 ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg
     2461 ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt
     2521 aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat
     2581 atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc
     2641 ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc
     2701 aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg
     2761 cccctctatg acctgctgct ggagatgctg gacgcccacc gcctacatgc gcccactagc
     2821 cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc gggctctact
     2881 tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca
     2941 gtctgagagc tccctggcgg aattcccaga gatgttagct gaaatcatca ctaatcagat
     3001 accaaaatat tcaaatggaa atatcaaaaa gcttctgttt catcaaaaat gactcgacct
     3061 aactgagtaa gctagcttgt tcgagtatta tggcattggg aaaactgttt ttcttgtacc
     3121 atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa
     3181 tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa
     3241 tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca
     3301 aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat
     3361 gaggagtaaa acactagatc cccaaacaag cttggaaacc atggggaatc acgaattctg
     3421 gatccgatac gtaacgcgtc tgcagcatgc gtggtaccgc ggccgcgttt aaaccacccc
     3481 gtgtctagag tcgacctgca ggcatgcaag cttcacgctg ccgcaagcac tcagggcgca
     3541 agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc
     3601 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc
     3661 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa
     3721 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     3781 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga tcatgagcgg
     3841 agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt
     3901 tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc tggagtttaa
     3961 tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact
     4021 atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg
     4081 gtatccaatt agagtctcat attcactctc aatccagatc tcgactctag tcgagggccc
     4141 atgggagctt ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4201 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4261 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4321 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4381 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4441 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4501 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4561 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4621 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     4681 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4741 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4801 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4861 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     4921 cgccttcttg acgagttctt ctgagcggga cccaagctag cttcgacgga tccccgatcg
     4981 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     5041 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     5101 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     5161 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     5221 actagatcgg gaattgccaa gcttatcgat gggaccatca tactcattgc tgatccatgt
     5281 agatttcccg gacatgaagc catttacaat tgaatatatc ctgccgccgc tgccgctttg
     5341 cacccggtgg agcttgcatg ttggtttcta cgcagaactg agccggttag gcggaattcc
     5401 tcgacccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta
     5461 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccggcc
     5521 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
     5581 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
     5641 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
     5701 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
     5761 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
     5821 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
     5881 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
     5941 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
     6001 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcgggcctc
     6061 ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa aatggcagcc
     6121 aagctcctaa cattttatta gagagcaggc tagttgctta gatacatgat cttcaggccg
     6181 ttatctgtca gggcaagcga aaattggcca tttatgacga ccaatgcccc gcagaagctc
     6241 ccatctttgc cgccatagac gccgcgcccc ccttttgggg tgtagaacat ccttttgcca
     6301 gatgtggaaa agaagttcgt tgtcccattg ttggcaatga cgtagtagcc ggcgaaagtg
     6361 cgagacccat ttgcgctata tataagccta cgatttccgt tgcgactatt gtcgtaattg
     6421 gatgaactat tatcgtagtt gctctcagag ttgtcgtaat ttgatggact attgtcgtaa
     6481 ttgcttatgg agttgtcgta gttgcttgga gaaatgtcgt agttggatgg ggagtagtca
     6541 tagggaagac gagcttcatc cactaaaaca attggcaggt cagcaagtgc ctgccccgat
     6601 gccatcgcaa gtacgaggct tagaaccacc ttcaacagat cgcgcatagt cttccccagc
     6661 tctctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg aattgttaga
     6721 cattatttgc cgactacctt ggtgatctcg cctttcacgt agtgaacaaa ttcttccaac
     6781 tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat aagcctgcct agcttcaagt
     6841 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     6901 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     6961 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     7021 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     7081 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     7141 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     7201 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     7261 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     7321 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     7381 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     7441 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     7501 gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc gcgaagcggt gtcggcttga
     7561 atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt accagtacat cgctgtttcg
     7621 ttcgagactt gaggtctagt tttatacgtg aacaggtcaa tgccgccgag agtaaagcca
     7681 cattttgcgt acaaattgca ggcaggtaca ttgttcgttt gtgtctctaa tcgtatgcca
     7741 aggagctgtc tgcttagtgc ccactttttc gcaaattcga tgagactgtg cgcgactcct
     7801 ttgcctcggt gcgtgtgcga cacaacaatg tgttcgatag aggctagatc gttccatgtt
     7861 gagttgagtt caatcttccc gacaagctct tggtcgatga atgcgccata gcaagcagag
     7921 tcttcatcag agtcatcatc cgagatgtaa tccttccggt aggggctcac acttctggta
     7981 gatagttcaa agccttggtc ggataggtgc acatcgaaca cttcacgaac aatgaaatgg
     8041 ttctcagcat ccaatgtttc cgccacctgc tcagggatca ccgaaatctt catatgacgc
     8101 ctaacgcctg gcacagcgga tcgcaaacct ggcgcggctt ttggcacaaa aggcgtgaca
     8161 ggtttgcgaa tccgttgctg ccacttgttt aatagactgg atggaggcgg ataaagttgc
     8221 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8281 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8341 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8401 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8461 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8521 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8581 ccccgtagaa aagatcaaag gatcttcttg atatcctttt tttctgcgcg taatctgctg
     8641 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8701 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     8761 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8821 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8881 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8941 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9001 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9061 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9121 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9181 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9241 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9301 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9361 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     9421 ttcacaccgc atatgaagat cggcggcaat agcttcttag cgccatcccg gctgagaaag
     9481 cccagtaagg aaacaactgt aggttcgagt cgcgagatcc cccggaacca aaggaagtag
     9541 gttaaacccg ctccgatcag gccgagccac gccaggccga gaacattggt tcctgtaggc
     9601 atcgggattg gcggatcaaa cactaaagct actggaacga gcagaagtcc tccggccgcc
     9661 agttgccagg cggtaaaggt gagcagaggc acgggaggtt gccacttgcg ggtcagcacg
     9721 gttccgaacg ccatggaaac cgcccccgcc aggcccgctg cgacgccgac aggatctagc
     9781 gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat agcccccagg
     9841 accgccatca atcgtatcgg gctacctagc agagcggcag agatgaacac gaccatcagc
     9901 ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca ggcggtagac cgaaataaac
     9961 aacaagctcc agaatagcga aatattaagt gcgccgagga tgaagatgcg catccaccag
    10021 attcccgttg gaatctgtcg gacgatcatc acgagcaata aacccgccgg caacgcccgc
    10081 agcagcatac cggcgacccc tcggcctcgc tgttcgggct ccacgaaaac gccggacaga
    10141 tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga agaccgacag cccaatgatc
    10201 tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca accgttcagc gaacgcctcc
    10261 atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca tctctggagc tttcttcagg
    10321 gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg ctccaagccg tcgaatctga
    10381 gccttaatca caattgtcaa ttttaatcct ctgtttatcg gcagttcgta gagcgcgccg
    10441 tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa
    10501 atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca aggccctagc
    10561 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
    10621 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
    10681 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
    10741 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
    10801 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
    10861 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
    10921 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
    10981 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
    11041 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
    11101 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
    11161 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
    11221 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
    11281 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
    11341 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
    11401 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
    11461 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
    11521 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg
    11581 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
    11641 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
    11701 tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct
    11761 gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg
    11821 tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga
    11881 ataccagctc cgcgaagtcg ctcttcttga tggagcgcat ggggacgtgc ttggcaatca
    11941 cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg gctctgccct cgggcggacc
    12001 acgcccatca tgaccttgcc aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg
    12061 aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc
    12121 aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac gtgctcatag
    12181 tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc cgacaggctc
    12241 atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc
    12301 ttgataggtg ggctgccctt cctggttggg taatgactcc aacttattga tagtgtttta
    12361 tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag aacgacagcg
    12421 acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc tcaattcgct
    12481 gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca tacagcggcc
    12541 agccatccgt catccatatc accacgtcaa agggtgacag caggctcata agacgcccca
    12601 gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg agactgtcat
    12661 acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac atagccccac tgttcgtcca
    12721 tttccgcgca gacgatgacg tcactgcccg gctgtatgcg cgaggttacc gactgcggcc
    12781 tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg cccataatgc gggctgttgc
    12841 ccggcatcca acgccattca tggccatatc aatgattttc tggtgcgtac cgggttgaga
    12901 agcggtgtaa gtgaactgca gttgccatgt tttacggcag tgagagcaga gatagcgctg
    12961 atgtccggcg gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca
    13021 gctgatagaa acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta
    13081 agttggcagc atcacccctg gttggcttgg tttcatcagc catccgcttg ccctcatctg
    13141 ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
    13201 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
    13261 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
    13321 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
    13381 agggttatgc agcggaaaag atccgtcgag gtcgagctgg atacttcccg tccgccaggg
    13441 ggacatgccg gcgatgctga aggtcgcgcg cattcccgat gaagaggccg gtaaccgcgt
    13501 tcggcgtgct ctccgatgtc gatagcggct atccgctgct gctgtcggaa atgacaatcc
    13561 tgacggcctt gcga
//
