LOCUS       sequence-369771-        3528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A14_STOP
FEATURES             Location/Qualifiers
     source          1..3528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1085..1184)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1201..1220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1202..1220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1225..1241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1225..1241)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1343..1362)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1354..2163
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1421..1440)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2310..2898
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2799..2818
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3052..3069
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3228..3255)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3347..3433)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3482..3504
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3496..3513
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3497..3513
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg catcttccca
      121 ggcatcatcc tgatcttcct gagggtgaag tttgcaaccg cagccgtgat cgtgagcgga
      181 caccagaagt ccaccacagt gtctcacgag atgagcggcc tgaactggaa gcctttcgtg
      241 tacggaggac tggcaagcat cgtggccgag ttcggcacat ttccagtgga cctgaccaag
      301 acacgcctgc aggtgcaggg ccagtccatc gatgccaggt ttaaggagat caagtaccgc
      361 ggcatgttcc acgccctgtt taggatctgc aaggaggagg gcgtgctggc cctgtatagc
      421 ggaatcgcac ccgccctgct gaggcaggcc tcctacggca ccatcaagat cggcatctat
      481 cagtctctga agcggctgtt tgtggagaga ctggaggacg agacactgct gatcaacatg
      541 atctgtggcg tggtgtctgg cgtgatcagc tccaccatcg ccaatcctac agacgtgctg
      601 aagatccgga tgcaggcaca gggaagcctg ttccagggat ccatgatcgg ctcttttatc
      661 gatatctacc agcaggaggg aaccagggga ctgtggaggg gagtggtgcc aacagcacag
      721 agagcagcaa tcgtggtggg agtggagctg cccgtgtatg acatcaccaa gaagcacctg
      781 atcctgtccg gcatgatggg cgataccatc ctgacacact tcgtgtctag ctttacctgc
      841 ggactggcag gcgccctggc atctaacccc gtggatgtgg tgcggacaag aatgatgaat
      901 cagagggcaa tcgtgggaca cgtggacctg tacaagggca ccgtggatgg catcctgaag
      961 atgtggaagc acgagggctt ctttgccctg tataagggat tctggccaaa ctggctgagg
     1021 ctgggacctt ggaacatcat cttctttatc acatacgagc agctgaagag actgcagatc
     1081 taagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1141 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1201 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1261 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1321 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1381 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1441 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1501 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1561 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1621 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1681 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1741 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1801 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     1861 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     1921 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     1981 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2041 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2101 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2161 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2221 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2281 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2341 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2401 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2461 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2521 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2581 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2641 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2701 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2761 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2821 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2881 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2941 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3001 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3061 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3121 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3181 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3241 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3301 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3361 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3421 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3481 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
